Antioxidant Potential of Lingzhi or Reishi Medicinal Mushroom, Ganoderma lucidum (Higher Basidiomycetes) Cultivated on Artocarpus heterophyllus Sawdust Substrate in India

Int J Med Mushrooms. 2015;17(12):1171-7. doi: 10.1615/intjmedmushrooms.v17.i12.70.


The artificial cultivation of Ganoderma lucidum (MTCC1039) using Artocarpus heterophyllus as sawdust substrate was optimized and free radical scavenging activities of the generated fruiting bodies were investigated. The choice of A. heterophyllus as substrate was due to its easy availability in South India. Sawdust supplemented with dextrose medium yielded better spawn hyphae and early fruiting body initiation (15 days). The biological yield obtained was 42.06 ± 2.14 g/packet and the biological efficiency was 8.41 ± 0.48%. Both aqueous and methanolic extracts of fruiting body were analyzed for radical scavenging activity. Methanolic extract showed maximum scavenging activity for 1,1-diphenyl-2-picrylhydrazyl (IC50 = 290 μg/ml) and 2,2'-azino-bis(3-ethylbenzothiazoline- 6-sulphonic acid (IC50 = 580 μg/ml), whereas aqueous extract had better scavenging for ferric reducing antioxidant power (IC50 = 5 μg/ml). Total phenolic content and total antioxidant capacity were significantly higher in methanolic extract (p < 0.01). A positive correlation existed between the phenolic content and antioxidant activity. Our results indicated that fruiting bodies of G. lucidum cultivated in sawdust medium possess antioxidant property, which can be exploited for therapeutic application.

MeSH terms

  • Antioxidants / pharmacology*
  • Artocarpus*
  • Benzothiazoles
  • Biphenyl Compounds
  • Carbon / metabolism
  • Ferric Compounds / metabolism
  • Free Radicals / metabolism
  • Fruiting Bodies, Fungal / growth & development
  • Mycelium / metabolism
  • Phenols / analysis
  • Phenols / pharmacology
  • Picrates
  • Reishi / chemistry*
  • Reishi / growth & development*
  • Sulfonic Acids
  • Wood


  • Antioxidants
  • Benzothiazoles
  • Biphenyl Compounds
  • Ferric Compounds
  • Free Radicals
  • Phenols
  • Picrates
  • Sulfonic Acids
  • 2,2'-azino-di-(3-ethylbenzothiazoline)-6-sulfonic acid
  • Carbon
  • 1,1-diphenyl-2-picrylhydrazyl