Localization of histamine (H1, H2, H3 and H4) receptors in mouse inner ear

Acta Otolaryngol. 2016 Jun;136(6):537-44. doi: 10.3109/00016489.2015.1136433. Epub 2016 Feb 6.


Conclusion The present findings show that all four types of histamine receptors (H1R, H2R, H3R, and H4R) are present in the inner ear, thus supporting the hypothesis that histamine plays a physiological role in the inner ear. Objective To analyse the presence of histamine receptors in the normal mouse inner ear. Methods CBA/J mice were used in this study. The localization of H1R, H2R, H3R, and H4R in the inner ear, i.e. cochlea, vestibular end organs, vestibular ganglion, and endolymphatic sac, was studied by real-time PCR and immunohistochemistry. Results The mRNA for each receptor sub-type was detected in the inner ear. In the immunohistochemical study, the organ of Corti, spiral ganglion, vestibular ganglion, vestibular sensory epithelium, and endolymphatic sac cells showed an immunofluorescent reaction to all histamine receptors.

Keywords: Histamine; RT-PCR; histamine receptor; immunohistochemistry; inner ear; mouse.

MeSH terms

  • Animals
  • Ear, Inner / metabolism*
  • Immunohistochemistry
  • Mice, Inbred CBA
  • Real-Time Polymerase Chain Reaction
  • Receptors, Histamine / metabolism*


  • Receptors, Histamine