Potential Therapeutic Applications of Mucuna pruriens Peptide Fractions Purified by High-Performance Liquid Chromatography as Angiotensin-Converting Enzyme Inhibitors, Antioxidants, Antithrombotic and Hypocholesterolemic Agents

J Med Food. 2016 Feb;19(2):187-95. doi: 10.1089/jmf.2015.0098.


A Mucuna pruriens protein concentrate was hydrolyzed with a digestive (pepsin-pancreatin) enzymatic system. The soluble portion of the hydrolysate was fractionated by ultrafiltration and the ultrafiltered peptide fraction (PF) with lower molecular weight was purified by reversed-phase high-performance liquid chromatography. The PF obtained were evaluated by testing the biological activity in vitro. Fractions showed that the ability to inhibit the angiotensin-converting enzyme had IC50 values that ranged from 2.7 to 6.2 μg/mL. Trolox equivalent antioxidant capacity values ranged from 132.20 to 507.43 mM/mg. The inhibition of human platelet aggregation ranged from 1.59% to 11.11%, and the inhibition of cholesterol micellar solubility ranged from 0.24% to 0.47%. Hydrophobicity, size, and amino acid sequence could be factors in determining the biological activity of peptides contained in fractions. This is the first report that M. pruriens peptides act as antihypertensives, antioxidants, and inhibitors for human platelet aggregation and cholesterol micellar solubility in vitro.

Keywords: Mucuna pruriens; biological activity; peptide fractions; protein hydrolysate; purification.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Angiotensin-Converting Enzyme Inhibitors / pharmacology*
  • Antioxidants / pharmacology*
  • Chromatography, High Pressure Liquid
  • Fibrinolytic Agents / pharmacology*
  • Humans
  • Hydrolysis
  • Inhibitory Concentration 50
  • Molecular Weight
  • Mucuna / chemistry*
  • Peptidyl-Dipeptidase A / blood
  • Plant Proteins / chemistry
  • Plant Proteins / pharmacology*
  • Platelet Aggregation / drug effects
  • Protein Hydrolysates / chemistry
  • Protein Hydrolysates / pharmacology


  • Angiotensin-Converting Enzyme Inhibitors
  • Antioxidants
  • Fibrinolytic Agents
  • Plant Proteins
  • Protein Hydrolysates
  • ACE protein, human
  • Peptidyl-Dipeptidase A