Kinetochore localization in micronucleated cytokinesis-blocked Chinese hamster ovary cells: a new and rapid assay for identifying aneuploidy-inducing agents

Mutat Res. 1989 Dec;224(4):517-25. doi: 10.1016/0165-1218(89)90079-7.

Abstract

We have developed a modified micronucleus assay using an antikinetochore antibody and cytokinesis-blocked Chinese hamster ovary cells as a simple and rapid method for detecting aneuploidy-inducing agents. The presence of a kinetochore in a micronucleus of a binucleated cell indicates a cell with a high probability for aneuploidy following cytokinesis. The method requires minimal training to perform and score and can readily distinguish aneuploidy-inducing agents from clastogens. Micronucleated cells treated with the aneuploidy-inducing agents benomyl and vinblastine sulfate contained a kinetochore-positive micronucleus 92% and 94% of the time whereas micronucleated cells treated with the clastogen methyl methanesulfonate contained a kinetochore-positive micronucleus only 11% of the time. This relatively simple method for distinguishing aneuploidy-inducing agents from clastogenic agents may be used as a routine genotoxicity assay to identify environmental and therapeutic agents with aneuploidy-inducing properties.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Aneuploidy*
  • Animals
  • Benomyl / toxicity
  • Cell Division / drug effects
  • Cell Line
  • Centromere / ultrastructure*
  • Chromosomes / ultrastructure*
  • Cricetinae
  • Cricetulus
  • Dose-Response Relationship, Drug
  • Female
  • Fluorescent Antibody Technique
  • Methyl Methanesulfonate / toxicity
  • Micronucleus Tests / methods*
  • Ovary
  • Vinblastine / toxicity

Substances

  • Vinblastine
  • Methyl Methanesulfonate
  • Benomyl