Data supporting the spectrophotometric method for the estimation of catalase activity

Mahmoud Hussein Hadwan; Hussein Najm Abed

doi:10.1016/j.dib.2015.12.012

Data supporting the spectrophotometric method for the estimation of catalase activity

Data Brief. 2015 Dec 17:6:194-9. doi: 10.1016/j.dib.2015.12.012. eCollection 2016 Mar.

Authors

Mahmoud Hussein Hadwan¹, Hussein Najm Abed²

Affiliations

¹ Chemistry Department, College of Science, University of Babylon, Babylon Governorate, P.O. 51002, Hilla City, Iraq.
² Chemistry Department, College of Science, Al-Qadisiyah University, Iraq.

Abstract

Here we provide raw and processed data and methods for the estimation of catalase activities. The method for presenting a simple and accurate colorimetric assay for catalase activities is described. This method is based on the reaction of undecomposed hydrogen peroxide with ammonium molybdate to produce a yellowish color, which has a maximum absorbance at 374 nm. The method is characterized by adding a correction factor to exclude the interference that arises from the presence of amino acids and proteins in serum. The assay acts to keep out the interferences that arose from measurement of absorbance at unsuitable wavelengths.

Keywords: Ammonium molybdate; Catalase activity; Hydrogen peroxide; Serum.