The one-pot multienzyme (OPME) synthesis of human blood group H antigens and a human milk oligosaccharide (HMOS) with highly active Thermosynechococcus elongates α1-2-fucosyltransferase

Chem Commun (Camb). 2016 Mar 11;52(20):3899-902. doi: 10.1039/c5cc10646j. Epub 2016 Feb 11.


A novel α1-2-fucosyltransferase from Thermosynechococcus elongatus BP-1 (Te2FT) with high fucosyltransferase activity and low donor hydrolysis activity was discovered and characterized. It was used in an efficient one-pot multienzyme (OPME) fucosylation system for the high-yield synthesis of human blood group H antigens containing β1-3-linked galactosides and an important human milk oligosaccharide (HMOS) lacto-N-fucopentaose I (LNFP I) on preparative and gram scales. LNFP I was shown to be selectively consumed by Bifidobacterium longum subsp. infantis but not Bifidobacterium animalis subsp. lactis and is a potential prebiotic.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Blood Group Antigens / biosynthesis*
  • Blood Group Antigens / chemistry
  • Carbohydrate Conformation
  • Cyanobacteria / enzymology*
  • Enzyme Activation
  • Fucosyltransferases / metabolism*
  • Galactoside 2-alpha-L-fucosyltransferase
  • Humans
  • Milk, Human / chemistry*
  • Milk, Human / metabolism
  • Oligosaccharides / biosynthesis*
  • Oligosaccharides / chemistry


  • Blood Group Antigens
  • Oligosaccharides
  • Fucosyltransferases