Solution and Solid-State Analysis of Binding of 13-Substituted Berberine Analogues to Human Telomeric G-quadruplexes

Chem Asian J. 2016 Apr 5;11(7):1107-15. doi: 10.1002/asia.201600116. Epub 2016 Mar 11.


The interaction between 13-phenylalkyl and 13-diphenylalkyl berberine derivatives (NAX) and human telomeric DNA G4 structures has been investigated by both spectroscopic and crystallographic methods. NAX042 and NAX053 are the best compounds improving the performance of the natural precursor berberine. This finding is in agreement with the X-ray diffraction result for the NAX053-Tel12 adduct, showing the ligand which interacts via π-stacking, sandwiched at the interface of two symmetry-related quadruplex units, with its benzhydryl group contributing to the overall stability of the adduct by means of additional π-stacking interactions with the DNA residues. The berberine derivatives were also investigated for their cytotoxic activity towards a panel of human cancer cell lines. Compounds NAX042 and NAX053 affect the viability of cancer cell lines in a dose-dependent manner.

Keywords: G-quadruplexes; X-ray diffraction; alkaloids; berberine; crystal structure; telomeric DNA.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antineoplastic Agents / chemical synthesis
  • Antineoplastic Agents / chemistry*
  • Antineoplastic Agents / pharmacology
  • Berberine / analogs & derivatives*
  • Berberine / chemistry*
  • Berberine / pharmacology
  • Cell Line, Tumor
  • Cell Proliferation / drug effects
  • Cell Survival / drug effects
  • Crystallography, X-Ray
  • Dose-Response Relationship, Drug
  • Drug Screening Assays, Antitumor
  • G-Quadruplexes*
  • Humans
  • Ligands
  • Models, Molecular
  • Molecular Structure
  • Solutions
  • Structure-Activity Relationship
  • Telomere / chemistry*


  • Antineoplastic Agents
  • Ligands
  • Solutions
  • Berberine