Inhibition of the FACT Complex Reduces Transcription from the Human Cytomegalovirus Major Immediate Early Promoter in Models of Lytic and Latent Replication

J Virol. 2016 Mar 28;90(8):4249-4253. doi: 10.1128/JVI.02501-15. Print 2016 Apr.


The successful colonization of the majority of the population by human cytomegalovirus is a direct result of the virus's ability to establish and, more specifically, reactivate from latency. The underlying cellular factors involved in viral reactivation remain unknown. Here, we show that the host complexfacilitateschromatintranscription (FACT) binds to the major immediate early promoter (MIEP) and that inhibition of this complex reduces MIEP transactivation, thus inhibiting viral reactivation.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Cytomegalovirus / genetics
  • Cytomegalovirus / physiology*
  • Fibroblasts
  • Gene Expression Regulation, Viral
  • Genes, Immediate-Early*
  • Humans
  • Models, Biological
  • Promoter Regions, Genetic
  • Transcription, Genetic
  • Viral Proteins / antagonists & inhibitors*
  • Viral Proteins / metabolism
  • Virus Latency
  • Virus Release
  • Virus Replication*


  • Viral Proteins