Expression and clinical significance of cyclooxygenase-2 and interleukin-32 in primary gastric B-cell lymphoma

Yingying Cui; Zhenchang Sun; Xin Li; Changsen Leng; Lei Zhang; Xiaorui Fu; Ling Li; Xudong Zhang; Y U Chang; Feifei Nan; Zhaoming Li; Jiaqin Yan; Mingzhi Zhang; Wencai Li; Guannan Wang; Dandan Zhang; Yaozhen Ma

doi:10.3892/ol.2015.3950

Expression and clinical significance of cyclooxygenase-2 and interleukin-32 in primary gastric B-cell lymphoma

Oncol Lett. 2016 Jan;11(1):693-698. doi: 10.3892/ol.2015.3950. Epub 2015 Nov 20.

Authors

Yingying Cui¹, Zhenchang Sun¹, Xin Li¹, Changsen Leng², Lei Zhang¹, Xiaorui Fu¹, Ling Li¹, Xudong Zhang¹, Y U Chang¹, Feifei Nan¹, Zhaoming Li¹, Jiaqin Yan¹, Mingzhi Zhang¹, Wencai Li³, Guannan Wang³, Dandan Zhang³, Yaozhen Ma¹

Affiliations

¹ Department of Oncology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan 450052, P.R. China.
² Department of Thoracic Surgery, The Affiliated Cancer Hospital of Zhengzhou University, Zhengzhou, Henan 450008, P.R. China.
³ Department of Pathology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan 450052, P.R. China.

Abstract

Cyclooxygenase-2 (COX-2) and interleukin-32 (IL-32) expression has been examined in various carcinomas and inflammations, and has been suggested to be significant in tumor progression and prognosis. The present study was conducted to investigate the expression of COX-2 and IL-32 in primary gastric B-cell lymphoma in order to define their clinical significance and their association with Helicobacter pylori (Hp) infection. COX-2 and IL-32 protein expression was detected in 31 primary gastric B-cell lymphoma patients and 19 chronic gastritis patients with immunohistochemistry. COX-2 and IL-32 expression was significantly higher in primary gastric lymphoma (PGL) tissues compared with gastritis tissues (51.6 vs. 21.1% for COX-2, P=0.032; and 58.1 vs. 26.3% for IL-32, P=0.029) and was significantly higher in Hp⁺ lymphoma tissues compared with Hp^- lymphoma tissues (66.7 vs. 20% for COX-2, P=0.015; and 71.4 vs. 30% for IL-32, P=0.029). In the PGL tissues, the expression level of COX-2 was positively correlated with the expression level of IL-32, and the two were each positively correlated with Hp infection (P=0.004 for COX-2 and IL-32; P=0.01 for COX-2 and Hp infection; and P=0.003 for IL-32 and Hp infection). COX-2 expression was found to be significantly associated (P<0.05) with an aggressive tumor type, higher expression of Ki-67, frequent lymph node metastasis and advanced stage. IL-32 expression was found to be significantly correlated (P<0.05) with frequent lymph node metastasis and an advanced stage. The survival time was longer in the COX-2^- and IL-32^- lymphoma patients compared with the COX-2⁺ and IL-32⁺ lymphoma patients, but these differences were not statistically significant. These results suggested that Hp infection and the expression of COX-2 and IL-32 were closely linked with each other, and that the overexpression of COX-2 and IL-32 was correlated with tumor progression in primary gastric B-cell lymphoma, thus indicating potential novel therapeutic target.

Keywords: Helicobacter pylori; cyclooxygenase-2; interleukin-32; metastasis; primary gastric lymphoma.