Nucleotide sequence of the Saccharomyces cerevisiae PUT4 proline-permease-encoding gene: similarities between CAN1, HIP1 and PUT4 permeases

Gene. 1989 Nov 15;83(1):153-9. doi: 10.1016/0378-1119(89)90413-7.


The complete nucleotide (nt) sequence of the PUT4 gene, whose product is required for high-affinity proline active transport in the yeast Saccharomyces cerevisiae, is presented. The sequence contains a single long open reading frame of 1881 nt, encoding a polypeptide with a calculated Mr of 68,795. The predicted protein is strongly hydrophobic and exhibits six potential glycosylation sites. Its hydropathy profile suggests the presence of twelve membrane-spanning regions flanked by hydrophilic N- and C-terminal domains. The N terminus does not resemble signal sequences found in secreted proteins. These features are characteristic of integral membrane proteins catalyzing translocation of ligands across cellular membranes. Protein sequence comparisons indicate strong resemblance to the arginine and histidine permeases of S. cerevisiae, but no marked sequence similarity to the proline permease of Escherichia coli or to other known prokaryotic or eukaryotic transport proteins. The strong similarity between the three yeast amino acid permeases suggests a common ancestor for the three proteins.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • ATP-Binding Cassette Transporters*
  • Amino Acid Sequence
  • Amino Acid Transport Systems*
  • Amino Acid Transport Systems, Basic*
  • Amino Acid Transport Systems, Neutral*
  • Bacterial Proteins*
  • Base Sequence
  • Membrane Transport Proteins / genetics*
  • Molecular Sequence Data
  • Restriction Mapping
  • Saccharomyces cerevisiae / genetics*
  • Saccharomyces cerevisiae Proteins
  • Sequence Homology, Nucleic Acid


  • ATP-Binding Cassette Transporters
  • Amino Acid Transport Systems
  • Amino Acid Transport Systems, Basic
  • Amino Acid Transport Systems, Neutral
  • Bacterial Proteins
  • CAN1 protein, S cerevisiae
  • Membrane Transport Proteins
  • Saccharomyces cerevisiae Proteins
  • arginine permease
  • histidine permease, Bacteria
  • proline transporter

Associated data

  • GENBANK/M30583