Haspin has Multiple Functions in the Plant Cell Division Regulatory Network

Plant Cell Physiol. 2016 Apr;57(4):848-61. doi: 10.1093/pcp/pcw030. Epub 2016 Feb 12.

Abstract

Progression of cell division is controlled by various mitotic kinases. In animal cells, phosphorylation of histone H3 at Thr3 by the kinase Haspin (haploid germ cell-specific nuclear protein kinase) promotes centromeric Aurora B localization to regulate chromosome segregation. However, less is known about the function of Haspin in regulatory networks in plant cells. Here, we show that inhibition of Haspin with 5-iodotubercidin (5-ITu) in Bright Yellow-2 (BY-2) cells delayed chromosome alignment. Haspin inhibition also prevented the centromeric localization of Aurora3 kinase (AUR3) and disrupted its function. This suggested that Haspin plays a role in the specific positioning of AUR3 on chromosomes in plant cells, a function conserved in animals. The results also indicated that Haspin and AUR3 are involved in the same pathway, which regulates chromosome alignment during prometaphase/metaphase. Remarkably, Haspin inhibition by 5-ITu also led to a severe cytokinesis defect, resulting in binuclear cells with a partially formed cell plate. The 5-ITu treatment did not affect microtubules, AUR1/2 or the NACK-PQR pathway; however, it did alter the distribution of actin filaments on the cell plate. Together, these results suggested that Haspin has several functions in regulating cell division in plant cells: in the localization of AUR3 on centromeres and in regulating late cell plate expansion during cytokinesis.

Keywords: Aurora3 kinase; BY-2; Chromosome alignment; Cytokinesis; Haspin.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aurora Kinases / metabolism
  • Cell Division
  • Centromere / metabolism
  • Chromosomes, Plant
  • Metabolic Networks and Pathways
  • Metaphase
  • Plant Cells / metabolism*
  • Plant Proteins / genetics
  • Plant Proteins / metabolism*
  • Plants, Genetically Modified
  • Protein Kinases / genetics
  • Protein Kinases / metabolism
  • Tobacco / cytology*
  • Tobacco / drug effects
  • Tubercidin / analogs & derivatives
  • Tubercidin / pharmacology

Substances

  • Plant Proteins
  • 5-iodotubercidin
  • Protein Kinases
  • Aurora Kinases
  • Tubercidin

Associated data

  • GENBANK/LC052296
  • GENBANK/LC052297
  • GENBANK/LC052298
  • GENBANK/LC052299