Herb-drug interaction of Epimedium extract on the pharmacokinetic of dapoxetine in rats

J Chromatogr B Analyt Technol Biomed Life Sci. 2016 Mar 1;1014:64-9. doi: 10.1016/j.jchromb.2016.02.001. Epub 2016 Feb 4.

Abstract

The aim of study is to develop a high performance liquid chromatography tandem mass spectrometry (LC-MS/MS) method to investigate the pharmacokinetic interaction of Epimedium extract on the dapoxetine in rats. Experimental rats were divided into the following four parallel groups: (1) dapoxetine alone (10mg/kg, i.v.); (2) oral administration of Epimedium extract (2g/kg) for 3 consecutive days and on the fourth day dapoxetine was administered (10mg/kg, i.v.); (3) dapoxetine alone (10mg/kg, p.o.); (4) oral administration of Epimedium extract (2g/kg) for 3 consecutive days and on the fourth day dapoxetine was administered (10mg/kg, p.o.). The calibration curves of dapoxetine were acquired over a concentration ranges from 1 to 500ng/mL with the R(2)=0.999. The mean matrix effects and extraction recoveries of dapoxetine at three different concentrations (1, 10, 500ng/mL) ranged from 107.3 to 110.9% and from 25.5 to 28.2% respectively. The interday and intraday relative standard deviation were both <6% while the bias were both <14%. The pharmacokinetic results demonstrated that pretreated with/without Epimedium extract for three consecutive days did not significant alter the pharmacokinetics of dapoxetine in rats. The oral bioavailability of dapoxetine was about 75% in rats.

Keywords: Dapoxetine; Epimedium sagittatum extract; Herbal drug interaction; Pharmacokinetics; Traditional Chinese medicine.

Publication types

  • Evaluation Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Benzylamines / administration & dosage
  • Benzylamines / blood*
  • Biological Availability
  • Chromatography, High Pressure Liquid / methods
  • Drugs, Chinese Herbal / administration & dosage
  • Drugs, Chinese Herbal / chemistry*
  • Drugs, Chinese Herbal / pharmacology*
  • Epimedium / chemistry*
  • Herb-Drug Interactions*
  • Limit of Detection
  • Male
  • Naphthalenes / administration & dosage
  • Naphthalenes / blood*
  • Rats
  • Rats, Sprague-Dawley
  • Serotonin Uptake Inhibitors / administration & dosage
  • Serotonin Uptake Inhibitors / blood*
  • Tandem Mass Spectrometry / methods

Substances

  • Benzylamines
  • Drugs, Chinese Herbal
  • Naphthalenes
  • Serotonin Uptake Inhibitors
  • dapoxetine