Inosine-5'-monophosphate is a candidate agent to resolve rigor mortis of skeletal muscle

Anim Sci J. 2016 Nov;87(11):1407-1412. doi: 10.1111/asj.12562. Epub 2016 Feb 15.

Abstract

The object of the present study was to reveal the action of inosine-5'-monophosphate (IMP) toward myofibrils in postmortem muscles. IMP solubilized isolated actomyosin within a narrow range of KCl concentration, 0.19-0.20 mol/L, because of the dissociation of actomyosin into actin and myosin, but it did not solubilize the proteins in myofibrils with 0.2 mol/L KCl. However, IMP could solubilize both proteins in myofibrils with 0.2 mol/L KCl in the presence of 1 m mol/L pyrophosphate or 1.0-3.3 m mol/L adenosine-5'-diphosphate (ADP). Thus, we presumed that pyrophosphate and ADP released thin filaments composed of actin, and thick filaments composed of myosin from restraints of myofibrils, and then both filaments were solubilized through the IMP-induced dissociation of actomyosin. Thus, we concluded that IMP is a candidate agent to resolve rigor mortis because of its ability to break the association between thick and thin filaments.

Keywords: ADP; IMP; myofibrils; pyrophosphate; rigor mortis resolution.

MeSH terms

  • Actins
  • Actomyosin
  • Adenosine Diphosphate
  • Animals
  • Chickens
  • Diphosphates
  • Dose-Response Relationship, Drug
  • Inosine Monophosphate / pharmacology*
  • Meat*
  • Muscle Proteins / metabolism*
  • Muscle, Skeletal / metabolism*
  • Myofibrils
  • Myosins
  • Postmortem Changes*
  • Potassium Chloride
  • Proteolysis / drug effects*
  • Solubility
  • Swine

Substances

  • Actins
  • Diphosphates
  • Muscle Proteins
  • Inosine Monophosphate
  • diphosphoric acid
  • Adenosine Diphosphate
  • Potassium Chloride
  • Actomyosin
  • Myosins