The ROS-generating oxidase Nox1 is required for epithelial restitution following colitis

Exp Anim. 2016 Jul 29;65(3):197-205. doi: 10.1538/expanim.15-0127. Epub 2016 Feb 15.


Accumulating evidence suggests that reactive oxygen species (ROS) generated by endogenous metabolic enzymes are involved in a variety of intracellular mechanisms. In particular, superoxide-generating NADPH oxidase (Nox) 1 is highly expressed in the colon and has been implicated in physiological and pathophysiological states of colon tissues. However, its role in tissue repair following colitis has not been fully elucidated. Our study using experimental colitis in mice showed that repair of the mucosal layer did not occur in Nox1-deficient mice following dextran sulfate sodium-induced colitis. This was accompanied by inhibition of proliferation, cell survival, migration, and terminal differentiation (generation of goblet cells) of crypt progenitor cells, as determined by histochemical analyses. Furthermore, Nox1 expression as well as ROS production in the colon crypt was increased during the repair process, and Nox1 deficiency suppressed these events. The results suggest that Nox1 promotes colon mucosal wound repair by sustaining the bioactivity of crypt progenitor cells and plays a crucial role in the epithelial restitution in the case of damage associated with colitis.

MeSH terms

  • Animals
  • Cell Movement
  • Cell Proliferation
  • Cell Survival
  • Colitis / chemically induced
  • Colitis / pathology*
  • Colitis / physiopathology*
  • Colon*
  • Dextran Sulfate
  • Gene Expression
  • Intestinal Mucosa / cytology
  • Intestinal Mucosa / enzymology*
  • Intestinal Mucosa / metabolism
  • Intestinal Mucosa / physiology*
  • Mice
  • NADH, NADPH Oxidoreductases / genetics
  • NADH, NADPH Oxidoreductases / metabolism*
  • NADH, NADPH Oxidoreductases / physiology*
  • NADPH Oxidase 1
  • Reactive Oxygen Species / metabolism*
  • Regeneration*


  • Reactive Oxygen Species
  • Dextran Sulfate
  • NADH, NADPH Oxidoreductases
  • NADPH Oxidase 1
  • NOX1 protein, mouse