Endothelial interleukin-8: a novel inhibitor of leukocyte-endothelial interactions

Science. 1989 Dec 22;246(4937):1601-3. doi: 10.1126/science.2688092.

Abstract

Certain inflammatory stimuli render cultured human vascular endothelial cells hyperadhesive for neutrophils. This state is transient and reversible, in part because activated endothelial cells secrete a leukocyte adhesion inhibitor (LAI). LAI was identified as endothelial interleukin-8 (IL-8), the predominant species of which is an extended amino-terminal IL-8 variant. At nanomolar concentrations, purified endothelial IL-8 and recombinant human IL-8 inhibit neutrophil adhesion to cytokine-activated endothelial monolayers and protect these monolayers from neutrophil-mediated damage. These findings suggest that endothelial-derived IL-8 may function to attenuate inflammatory events at the interface between vessel wall and blood.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Biological Factors / pharmacology
  • Cell Adhesion / drug effects
  • Cells, Cultured
  • Chemotactic Factors / isolation & purification*
  • Chemotactic Factors / pharmacology
  • Culture Media / analysis
  • Cytokines
  • Endothelium, Vascular / cytology
  • Endothelium, Vascular / drug effects
  • Endothelium, Vascular / physiology*
  • Humans
  • Interleukin-1 / pharmacology*
  • Interleukin-8
  • Interleukins / isolation & purification*
  • Interleukins / pharmacology
  • Molecular Sequence Data
  • Neutrophils / cytology
  • Neutrophils / drug effects
  • Neutrophils / physiology*
  • Recombinant Proteins / pharmacology

Substances

  • Biological Factors
  • Chemotactic Factors
  • Culture Media
  • Cytokines
  • Interleukin-1
  • Interleukin-8
  • Interleukins
  • Recombinant Proteins