Abstract
Certain inflammatory stimuli render cultured human vascular endothelial cells hyperadhesive for neutrophils. This state is transient and reversible, in part because activated endothelial cells secrete a leukocyte adhesion inhibitor (LAI). LAI was identified as endothelial interleukin-8 (IL-8), the predominant species of which is an extended amino-terminal IL-8 variant. At nanomolar concentrations, purified endothelial IL-8 and recombinant human IL-8 inhibit neutrophil adhesion to cytokine-activated endothelial monolayers and protect these monolayers from neutrophil-mediated damage. These findings suggest that endothelial-derived IL-8 may function to attenuate inflammatory events at the interface between vessel wall and blood.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Amino Acid Sequence
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Biological Factors / pharmacology
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Cell Adhesion / drug effects
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Cells, Cultured
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Chemotactic Factors / isolation & purification*
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Chemotactic Factors / pharmacology
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Culture Media / analysis
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Cytokines
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Endothelium, Vascular / cytology
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Endothelium, Vascular / drug effects
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Endothelium, Vascular / physiology*
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Humans
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Interleukin-1 / pharmacology*
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Interleukin-8
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Interleukins / isolation & purification*
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Interleukins / pharmacology
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Molecular Sequence Data
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Neutrophils / cytology
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Neutrophils / drug effects
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Neutrophils / physiology*
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Recombinant Proteins / pharmacology
Substances
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Biological Factors
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Chemotactic Factors
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Culture Media
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Cytokines
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Interleukin-1
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Interleukin-8
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Interleukins
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Recombinant Proteins