Schizocalyx cuspidatus (A. St.-Hil.) Kainul. & B. Bremer extract improves antioxidant defenses and accelerates the regression of hepatic fibrosis after exposure to carbon tetrachloride in rats

Reggiani Vilela Gonçalves; Rômulo Dias Novaes; Mariáurea Matias Sarandy; João Paulo Viana Leite; Emerson Ferreira Vilela; Marli do Carmo Cupertino; Sérgio Luis Pinto da Matta

doi:10.1080/14786419.2016.1143825

Schizocalyx cuspidatus (A. St.-Hil.) Kainul. & B. Bremer extract improves antioxidant defenses and accelerates the regression of hepatic fibrosis after exposure to carbon tetrachloride in rats

Nat Prod Res. 2016 Dec;30(23):2738-2742. doi: 10.1080/14786419.2016.1143825. Epub 2016 Feb 17.

Authors

Reggiani Vilela Gonçalves¹, Rômulo Dias Novaes², Mariáurea Matias Sarandy³, João Paulo Viana Leite⁴, Emerson Ferreira Vilela⁵, Marli do Carmo Cupertino³, Sérgio Luis Pinto da Matta³

Affiliations

¹ a Department of Animal Biology , Federal University of Viçosa , Viçosa , Brazil.
² b Department of Structural Biology, Institute of Biomedical Sciences , Federal University of Alfenas , Alfenas , Brazil.
³ c Department of General Biology , Federal University of Viçosa , Viçosa , Brazil.
⁴ d Department of Biochemistry and Molecular Biology , Federal University of Viçosa , Viçosa , Brazil.
⁵ e Department of Fitotecnia , Federal University of Viçosa , Viçosa , Brazil.

PMID: 26885892
DOI: 10.1080/14786419.2016.1143825

Abstract

The aim of this study was to investigate the effect of leaves extract of Schizocalyx cuspidatus (A. St.-Hil.) Kainul. & B. Bremer on hepatic morphofunctional dysfunction induced by carbon tetrachloride (CCl₄). Liver lesions were induced via intraperitoneal administration of CCl₄ every 48 h for 12 days. Forty-nine rats were randomised into seven groups: G1: CCl₄; G2: CCl₄ (animals euthanised 24 h after last CCl₄ application); G3: CCl₄ + DMSO; G4: SCE 400 mg/kg; G5: DMSO (700 μl); G6: CCl₄ + SCE 200 mg/kg and G7: CCl₄ + SCE 400 mg/kg. SCE administration resulted in reduction in hydroperoxide levels, lipidic droplets and necrosis compared to G1, G2 and G3. There was an increase in the amount of collagen fibres in G1, G2 and G3 compared to the groups. These results show that the extract of SCE leaves has great potential for the recovery of liver damage after the application of CCl₄.

Keywords: Fibrosis; antioxidant activity; carbon tetrachloride; hepatotoxicity.