Evaluation of biochemical and clinical markers of endothelial dysfunction and their correlation with urinary albumin excretion in patients with type 1 diabetes mellitus

Arch Endocrinol Metab. 2016 Apr;60(2):117-24. doi: 10.1590/2359-3997000000116. Epub 2016 Feb 16.

Abstract

Objective Endothelial dysfunction (ED) plays an important role in the pathogenesis of diabetic nephropathy. The purpose of the study was to determine flow mediated endothelial dependent vasodilatation (FMD) measurements and serum soluble (s) endothelin-1 (ET-1), intercellular adhesion molecule-1 (ICAM-1) and vascular cell adhesion molecule (VCAM-1) levels in patients with type 1 diabetes mellitus (T1DM) with or without increased urinary albumin excretion (UAE) and compare them with the healthy controls. Subjects and methods Seventy three patients with T1DM were enrolled. Patients were divided into two subgroups according to microalbumin measurements in 24-hr urine collections. The diabetic patients without microalbuminuria (41 patients) were defined as Group I and those with microalbuminuria (32 patients) were defined as group II. A hundred age and sex matched healthy subjects participated as the control group (Group III). Serum sET-1, sICAM-1, sVCAM-1 levels and FMD measurements were determined in all participants. Results Median FMD measurement was significantly lower in the diabetic groups compared with the control group (6.6, 6.4 and 7.8% in Group I, II and III, respectively) (p < 0.05). FMD was negatively correlated with age (p = 0.042). Median serum sICAM-1 level was higher in the patient groups compared to the control group (p < 0.05). Median serum sVCAM-1 level was higher in the group of patients with increased albuminuria compared to the normoalbuinuric and the control group (p < 0.05). Serum sVCAM-1 level was found to be positively correlated with degree of urinary albumin excretion (p < 0.001). Conclusion We assume that sVCAM-1 may be used as a predictive marker for risk stratification for nephropathy development and progression.

MeSH terms

  • Adult
  • Albuminuria / physiopathology*
  • Analysis of Variance
  • Biomarkers / blood
  • Blood Flow Velocity / physiology
  • Case-Control Studies
  • Diabetes Mellitus, Type 1 / blood*
  • Diabetes Mellitus, Type 1 / physiopathology*
  • Diabetic Nephropathies / blood
  • Diabetic Nephropathies / physiopathology
  • Endothelin-1 / blood
  • Endothelium, Vascular / physiopathology*
  • Female
  • Humans
  • Intercellular Adhesion Molecule-1 / blood
  • Male
  • Predictive Value of Tests
  • Reference Values
  • Risk Factors
  • Statistics, Nonparametric
  • Vascular Cell Adhesion Molecule-1 / blood
  • Vasodilation / physiology*

Substances

  • Biomarkers
  • Endothelin-1
  • Vascular Cell Adhesion Molecule-1
  • Intercellular Adhesion Molecule-1