Effect of Human Umbilical Cord Mesenchymal Stem Cell Transplantation in a Rat Model of Preeclampsia

Reprod Sci. 2016 Aug;23(8):1058-70. doi: 10.1177/1933719116630417. Epub 2016 Feb 17.


Objective: To test the effects of human umbilical cord mesenchymal stem cell (HU-MSC) transplantation on reversing preeclampsia (PE) symptoms in a lipopolysaccharide (LPS)-induced rat PE model.

Methods: Human umbilical cord MSCs were detected, isolated, and cultured. Human umbilical cord MSC transplantation was conducted. Expressions of inflammatory cytokines in serum and placental tissue were measured by enzyme-linked immunosorbent assay. Changes in inflammatory cytokines, peroxisome proliferator-activated receptor γ (PPARγ), laminin receptor 1 (LR1), matrix metalloproteinase (MMP) 2, and MMP-9 messenger RNA (mRNA) levels in placental tissue were recorded by quantitative real-time polymerase chain reaction. Immunohistochemistry and Western blotting were performed for PPARγ detection.

Results: The LPS group exhibited increased blood pressure and proteinuria and decreased fetal weight compared to the normal pregnancy (NP) group (all P < .05). The LPS + MSC group presented lowered blood pressure and higher fetal weight than the LPS group (P < .05). The levels of interferon γ, tumor necrosis factor α (TNF-α), interleukin (IL) 1β, IL-6, IL-8, IL-12, and intercellular adhesion molecule 1 (ICAM-1) increased and the levels of IL-4 and IL-10 levels decreased in the LPS group compared to the NP group (all P < .05). Tumor necrosis factor α, IL-6, IL-12, and ICAM-1 levels decreased and IL-10 level increased in the LPS + MSC group compared to the LPS group (all P < .05). The LPS-MSC group showed lower mRNA expressions of TNF-α, IL-6, MMP-2, MMP-9, and ICAM-1 and higher mRNA expressions of IL-10, PPARγ, and LR1 than the LPS group (all P < .05).

Conclusion: In summary, HU-MSC transplantation may be extremely beneficial for PE therapy.

Keywords: gestational hypertension; human umbilical cord mesenchymal stem cells; preeclampsia.

MeSH terms

  • Animals
  • Blood Pressure
  • Cord Blood Stem Cell Transplantation*
  • Cytokines / metabolism
  • Disease Models, Animal
  • Female
  • Humans
  • Inflammation / chemically induced
  • Inflammation / metabolism
  • Lipopolysaccharides
  • Mesenchymal Stem Cells / cytology
  • Mesenchymal Stem Cells / physiology
  • PPAR gamma / metabolism
  • Placenta / pathology
  • Pre-Eclampsia / chemically induced
  • Pre-Eclampsia / metabolism*
  • Pre-Eclampsia / pathology
  • Pre-Eclampsia / therapy*
  • Pregnancy
  • Proteinuria / etiology
  • RNA, Messenger / metabolism
  • Rats


  • Cytokines
  • Lipopolysaccharides
  • PPAR gamma
  • PPAR gamma, rat
  • RNA, Messenger