The v-myb oncogene product binds to and activates the promyelocyte-specific mim-1 gene

Cell. 1989 Dec 22;59(6):1115-25. doi: 10.1016/0092-8674(89)90767-8.


The v-myb oncogene induces myeloid leukemias in chickens, transforms myeloid cells in vitro, and encodes a sequence-specific DNA binding protein. We used differential hybridization to screen for v-myb-regulated genes in cells transformed by a temperature-sensitive mutant of the oncogene and identified a new gene, mim-1, which encodes a specifically expressed, secretable protein contained in the granules of both normal and v-myb-transformed promyelocytes. The promoter of the mim-1 gene contains three closely spaced binding sites for v-myb protein and is strongly activated by v-myb in a cotransfection assay. Synthetic copies of the binding sites are both necessary and sufficient to confer v-myb protein-dependent activation to a heterologous promoter. We conclude that mim-1 is a cellular gene that is directly regulated by the product of the v-myb oncogene.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Avian Myeloblastosis Virus / enzymology
  • Avian Myeloblastosis Virus / genetics
  • Base Sequence
  • Bone Marrow Cells
  • Cells, Cultured
  • Chickens
  • Cloning, Molecular
  • Deoxyribonuclease I
  • Escherichia coli / genetics
  • Gene Expression Regulation, Neoplastic*
  • Gene Library
  • Genes, Regulator*
  • Molecular Sequence Data
  • Oncogene Proteins v-myb
  • Oncogenes*
  • Promoter Regions, Genetic
  • Protein Binding
  • Protein-Tyrosine Kinases / metabolism
  • RNA, Messenger / genetics
  • Repetitive Sequences, Nucleic Acid
  • Restriction Mapping
  • Retroviridae Proteins, Oncogenic / genetics
  • Retroviridae Proteins, Oncogenic / metabolism*
  • Transcriptional Activation


  • Oncogene Proteins v-myb
  • RNA, Messenger
  • Retroviridae Proteins, Oncogenic
  • Protein-Tyrosine Kinases
  • Deoxyribonuclease I

Associated data

  • GENBANK/M29448
  • GENBANK/M29449