Direct expression cloning of vascular cell adhesion molecule 1, a cytokine-induced endothelial protein that binds to lymphocytes

Cell. 1989 Dec 22;59(6):1203-11. doi: 10.1016/0092-8674(89)90775-7.


We have cloned a previously undescribed adhesion molecule, VCAM-1, which is induced by cytokines on human endothelial cells and binds lymphocytes. Using a novel method requiring neither monoclonal antibodies nor purified protein, VCAM-1-expressing clones were selected by adhesion to human lymphoid cell lines. VCAM-1 mRNA is present in endothelial cells at 2 hr after treatment with IL-1 or TNF-alpha and is maintained for at least 72 hr; leukocyte binding activity parallels mRNA induction. Cells transfected with VCAM-1 bind the human leukemia lines Jurkat, Ramos, Raji, HL60, and THP1, but not peripheral blood neutrophils. VCAM-1, which belongs to the immunoglobulin gene super-family, may be central to recruitment of mononuclear leukocytes into inflammatory sites in vivo.

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • Biological Factors / pharmacology*
  • Cell Adhesion
  • Cell Adhesion Molecules / genetics*
  • Cell Adhesion Molecules / metabolism
  • Cell Line
  • Cells, Cultured
  • Cloning, Molecular*
  • Cytokines
  • DNA / genetics
  • DNA / isolation & purification
  • Endothelium, Vascular / drug effects
  • Endothelium, Vascular / metabolism*
  • Gene Expression / drug effects*
  • Gene Library
  • Genes*
  • Humans
  • Interleukin-1 / pharmacology*
  • Lymphocytes / metabolism*
  • Molecular Sequence Data
  • Protein Binding
  • Transfection
  • Tumor Necrosis Factor-alpha / pharmacology*


  • Biological Factors
  • Cell Adhesion Molecules
  • Cytokines
  • Interleukin-1
  • Tumor Necrosis Factor-alpha
  • DNA

Associated data

  • GENBANK/M30257