Glucose-induced oscillations of intracellular Ca2+ concentration resembling bursting electrical activity in single mouse islets of Langerhans

FEBS Lett. 1989 Dec 18;259(1):19-23. doi: 10.1016/0014-5793(89)81484-x.


Intracellular Ca2+ levels were monitored in single, acutely isolated mouse islets of Langerhans by dual emission Indo-1 fluorometry. High-frequency (3.1 min-1) [Ca2+]i oscillations with a brief rising time (1-2 s) and 10 s half-width ('fast' oscillations) were detected in 11 mM glucose. Raising the glucose concentration to 16.7 mM increased the duration of these oscillations, which were otherwise absent in 5.5 mM glucose. [Ca2+]i waves of lower frequency (0.5 min-1) and longer rising time ('slow' oscillations) were also recorded. The data indicate that "fast" oscillations are directly related to beta-cell bursting electrical activity, and suggest the existence of extensive networks of electrically coupled cells in the islet.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Calcium / metabolism*
  • Cytoplasm / metabolism
  • Dose-Response Relationship, Drug
  • Electrophysiology
  • Extracellular Space / physiology
  • Glucose / pharmacology*
  • In Vitro Techniques
  • Islets of Langerhans / physiology*
  • Mice
  • Periodicity


  • Glucose
  • Calcium