Mesenchymal stem cells (MSCs) possess self-proliferation and multi-directional differentiation abilities. Previous studies on MSCs have mostly focused on the bone marrow, lungs, pancreas and umbilical cord blood, with few studies on metanephric tissues in ducks. For the present study, the Beijing duck was selected as an experimental animal. Duck embryo metanephric mesenchymal stem cells (MMSCs) were studied. MMSC isolation culture, analysis of biological characteristics, induced differentiation and identification were performed in preliminary experiments. In the current study, surface antigens and gene expression patterns were detected using immunofluorescence, reverse transcription-polymerase chain reaction (RT-PCR) and flow cytometry. The induced cells, adipocytes, hepatocytes, epithelial cells and islet cells were identified by oil red O staining, periodic acid-Schiff staining, immunofluorescence and dithizone staining, respectively. RT-PCR was performed for detection of specific marker genes. The results suggested that the biological characteristics of MMSCs were similar to those of the MSCs previously analyzed. Primary MMSCs were sub-cultured to passage 21. The induced cells exhibit typical staining and immunofluorescence indicating the expression of specific genes. This demonstrates that MMSCs may be a novel alternative source of MSCs for experimental and clinical applications.
Keywords: Beijing duck; differentiation; metanephric mesenchymal stem cells.
Biological characterization of sheep kidney-derived mesenchymal stem cells.Exp Ther Med. 2016 Dec;12(6):3963-3971. doi: 10.3892/etm.2016.3902. Epub 2016 Nov 15. Exp Ther Med. 2016. PMID: 28105130 Free PMC article.
Differentiation potential of bone marrow mesenchymal stem cells in duck.J Genet Genomics. 2009 Mar;36(3):133-40. doi: 10.1016/S1673-8527(08)60100-9. J Genet Genomics. 2009. PMID: 19302969
Isolation, characterization, and in vitro proliferation of canine mesenchymal stem cells derived from bone marrow, adipose tissue, muscle, and periosteum.Am J Vet Res. 2012 Aug;73(8):1305-17. doi: 10.2460/ajvr.73.8.1305. Am J Vet Res. 2012. PMID: 22849692
Mesenchymal stem cells in rabbit meniscus and bone marrow exhibit a similar feature but a heterogeneous multi-differentiation potential: superiority of meniscus as a cell source for meniscus repair.BMC Musculoskelet Disord. 2015 Mar 21;16:65. doi: 10.1186/s12891-015-0511-8. BMC Musculoskelet Disord. 2015. PMID: 25887689 Free PMC article.
Stage-specific embryonic antigen 4 in Wharton's jelly-derived mesenchymal stem cells is not a marker for proliferation and multipotency.Tissue Eng Part A. 2014 Apr;20(7-8):1314-24. doi: 10.1089/ten.TEA.2013.0333. Epub 2014 Mar 14. Tissue Eng Part A. 2014. PMID: 24279891