The Yeast SWI4 Protein Contains a Motif Present in Developmental Regulators and Is Part of a Complex Involved in Cell-Cycle-Dependent Transcription

Nature. 1989 Dec 14;342(6251):830-3. doi: 10.1038/342830a0.

Abstract

Transcription of the HO gene of Saccharomyces cerevisiae, which encodes a site-specific endonuclease that initiates cell-type switching (reviewed in refs. 1,2), is restricted to a short window of the cell cycle in late G1 (refs 3,4). A repeated element in the upstream region of HO (the cell-cycle box, CCB) and two regulatory proteins, SWI4 and SWI6, are required for cell-cycle-dependent expression of HO. Biochemical experiments have identified a factor, CCBF (cell-cycle box factor), that binds to the CCB elements and that presumably plays a key part in cell-cycle regulation of HO. The SWI4 and SWI6 genes are required for formation of the CCBF-DNA complex. Here we report the nucleotide sequence of the SWI4 gene and show that it contains two copies of the conserved SWI6-cdc10 motif observed in SWI6 of budding yeast, the Schizosaccharomyces pombe cdc10 gene required for progression through G19, the Drosophila Notch gene, and in the Caenorhabditis elegans lin-12 and glp-1 genes. We demonstrate by using antibodies to the SWI4 protein in gel-shift assays that the protein is present in the CCBF-DNA complex.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • Cell Cycle*
  • DNA-Binding Proteins / metabolism
  • Fungal Proteins / genetics*
  • Gene Expression Regulation, Fungal*
  • Immunologic Techniques
  • Macromolecular Substances
  • Molecular Sequence Data
  • Regulatory Sequences, Nucleic Acid
  • Saccharomyces cerevisiae Proteins*
  • Transcription Factors / genetics*
  • Transcription, Genetic

Substances

  • DNA-Binding Proteins
  • Fungal Proteins
  • Macromolecular Substances
  • SWI4 protein, S cerevisiae
  • Saccharomyces cerevisiae Proteins
  • Transcription Factors