RNA-Seq Reveals the Angiogenesis Diversity between the Fetal and Adults Bone Mesenchyme Stem Cell

PLoS One. 2016 Feb 22;11(2):e0149171. doi: 10.1371/journal.pone.0149171. eCollection 2016.

Abstract

In this research, we used RNA sequencing (RNA-seq) to analyze 23 single cell samples and 2 bulk cells sample from human adult bone mesenchyme stem cell line and human fetal bone mesenchyme stem cell line. The results from the research demonstrated that there were big differences between two cell lines. Adult bone mesenchyme stem cell lines showed a strong trend on the blood vessel differentiation and cell motion, 48/49 vascular related differential expressed genes showed higher expression in adult bone mesenchyme stem cell lines (Abmsc) than fetal bone mesenchyme stem cell lines (Fbmsc). 96/106 cell motion related genes showed the same tendency. Further analysis showed that genes like ANGPT1, VEGFA, FGF2, PDGFB and PDGFRA showed higher expression in Abmsc. This work showed cell heterogeneity between human adult bone mesenchyme stem cell line and human fetal bone mesenchyme stem cell line. Also the work may give an indication that Abmsc had a better potency than Fbmsc in the future vascular related application.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adolescent
  • Adult
  • Adult Stem Cells / cytology
  • Adult Stem Cells / metabolism*
  • Cells, Cultured
  • Female
  • Fetus / cytology
  • Fetus / metabolism*
  • Gene Expression Regulation*
  • Humans
  • Male
  • Mesenchymal Stem Cells / cytology
  • Mesenchymal Stem Cells / metabolism*
  • Middle Aged
  • Neovascularization, Physiologic*

Grant support

XZ was supported by a PhD studentship from the BGI Education Center, University of Chinese Academy of Sciences, and the authors thank the support of the Shenzhen Municipal of Government of China (CXB201108250094A). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.