microRNA-34a-Upregulated Retinoic Acid-Inducible Gene-I Promotes Apoptosis and Delays Cell Cycle Transition in Cervical Cancer Cells

DNA Cell Biol. 2016 Jun;35(6):267-79. doi: 10.1089/dna.2015.3130. Epub 2016 Feb 24.

Abstract

The function of retinoic acid-inducible gene-I (RIG-I) in viral replication is well documented, but its function in carcinogenesis and malignancies as well as relationship with microRNAs (miRNAs) remain poorly understood. miR-34a is an antioncogene in multiple tumors. In our study, RIG-I and miR-34a suppressed cell growth, proliferation, migration, and invasion in cervical cancer cells in vitro. miR-34a was validated as a new regulator of RIG-I by binding to its 3' untranslated region and upregulating its expression level. Furthermore, we revealed that RIG-I and miR-34a enhanced apoptosis, delayed the G1/S/G2 transition of the cell cycle, and inhibited the epithelial-mesenchymal transition process to modulate malignancies in cervical cancer cells. Phenotypic rescue experiments indicated that RIG-I mediates the effects of miR-34a in HeLa and C33A cells. These findings provide new insights into the mechanisms that underlie carcinogenesis and may provide new biomarkers for the diagnosis and therapy of cervical cancer.

Publication types

  • Retracted Publication

MeSH terms

  • 3' Untranslated Regions
  • DEAD Box Protein 58 / genetics*
  • Female
  • Gene Expression Regulation*
  • Genes, Tumor Suppressor
  • HeLa Cells
  • Humans
  • MicroRNAs / metabolism*
  • Neoplasm Invasiveness
  • Receptors, Immunologic
  • Uterine Cervical Neoplasms / genetics*
  • Uterine Cervical Neoplasms / pathology

Substances

  • 3' Untranslated Regions
  • MIRN34 microRNA, human
  • MicroRNAs
  • Receptors, Immunologic
  • DDX58 protein, human
  • DEAD Box Protein 58