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. 2016 Feb 24;17:132.
doi: 10.1186/s12864-016-2470-3.

Identification and Expression Analysis of Genes Related to Calyx Persistence in Korla Fragrant Pear

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Free PMC article

Identification and Expression Analysis of Genes Related to Calyx Persistence in Korla Fragrant Pear

Maosong Pei et al. BMC Genomics. .
Free PMC article

Abstract

Background: The objective of this study was to increase understanding about genetic mechanisms affecting calyx persistence in Korla fragrant pear (Pyrus brestschneideri Rehd). Flowers were collected at early bloom, full bloom, and late bloom. The RNA was extracted from the flowers and then combined according to calyx type. Transcriptome and digital gene expression (DGE) profiles of flowers, ovaries, and sepals with persistent calyx (SC_hua, SC_ep, and SC_zf, respectively) were compared with those of flowers, ovaries, and sepals with deciduous calyx (TL_hua, TL_ep, and TL_zf, respectively). Temporal changes in the expression of selected genes in floral organs with either persistent or deciduous calyx were compared using real-time quantitative PCR (qRT-PCR).

Results: Comparison of the transcriptome sequences for SC_hua and TL_hua indicated 26 differentially expressed genes (DEGs) with known relationship to abscission and 10 DEGs with unknown function. We identified 98 MYB and 21 SPL genes from the assembled unigenes. From SC_zf vs TL_zf, we identified 21 DEGs with known relationship to abscission and 18 DEGs with unknown function. From SC_ep vs TL_ep, 12 DEGs with known relationship to abscission were identified along with 11 DEGs with unknown function. Ten DEGs were identified by both transcriptome sequencing and DGE sequencing.

Conclusions: More than 50 DEGs were observed that were related to calyx persistence in Korla fragrant pear. Some of the genes were related to cell wall degradation, plant hormone signal transduction, and stress response. Other DEGs were identified as zinc finger protein genes and lipid transfer protein genes. Further analysis showed that calyx persistence in Korla fragment pear was a metabolic process regulated by many genes related to cell wall degradation and plant hormones.

Figures

Fig. 1
Fig. 1
Length distribution of the assembled unigenes
Fig. 2
Fig. 2
GO categorization of unigenes
Fig. 3
Fig. 3
KOG annotation of putative proteins
Fig. 4
Fig. 4
KEGG annotation of putative proteins
Fig. 5
Fig. 5
Up-regulated and down-regulated differentially expressed genes in SC_hua vs TL_hua
Fig. 6
Fig. 6
Venn diagram of DEGs from SC_ep vs TL_ep and SC_zf vs TL_zf
Fig. 7
Fig. 7
Temporal changes in the expression of selected genes in complete flowers, ovaries, and sepals. Error bars indicate SD. Different lowercase letters within a panel indicate significant differences at P = 0.05. Different uppercase letters within a panel indicate significant differences at P = 0.01
Fig. 8
Fig. 8
Flowers with persistent and deciduous calyx of Korla fragrant pear. The a and b indicate flowers with persistent calyx. The c and d indicate flowers with deciduous calyx

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