Mechanism of Taiwan Mingjian Oolong Tea to Inhibit Isoproterenol-Induced Hypertrophy and Apoptosis in Cardiomyoblasts

Am J Chin Med. 2016;44(1):77-86. doi: 10.1142/S0192415X16500051.


This study investigates the cardio-protective effect of Nos. 1 and 5 extracts from Taiwan Mingjian Oolong Tea on H9c2 cardiomyoblast cells treated with isoproterenol (ISO). Treatment with Nos. 1 and 5 extracts increased cell viability and blocked apoptosis in ISO exposed H9c2 cells. Moreover, Nos. 1 and 5 extracts blocked hypertrophy markers like G[Formula: see text]s, calcineurin, NFATc3, and BNP, thereby increasing cell proliferation markers -PI3K and AKT in a dose dependent manner. In contrast, apoptotic proteins, such as caspase-3 and cytochrome c were decreased in H9c2 cells treated with Nos. 1 and 5 extracts. We confirmed that the protective effect of No. 1 extract was partially mediated through the expression of ERK and p38, however, the No. 5 extract showed a protective effect via the ERK, JNK, and p38 pathways. This evidence provides new insights into the pharmacological role and therapeutic mechanism of Taiwan Mingjian Oolong Tea in heart diseases.

Keywords: Cardiac Apoptosis; Cardiac Hypertrophy; Isoproterenol; Oolong Tea.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Apoptosis / drug effects*
  • Camellia sinensis / chemistry*
  • Cardiotonic Agents / pharmacology*
  • Cell Proliferation / drug effects
  • Cell Survival / drug effects
  • Cells, Cultured
  • Hypertrophy
  • Isoproterenol / adverse effects*
  • MAP Kinase Signaling System
  • Myocytes, Cardiac / cytology
  • Myocytes, Cardiac / drug effects*
  • Plant Extracts / pharmacology*
  • Rats
  • Taiwan
  • Tea / chemistry*
  • p38 Mitogen-Activated Protein Kinases


  • Cardiotonic Agents
  • Plant Extracts
  • Tea
  • p38 Mitogen-Activated Protein Kinases
  • Isoproterenol