Oxidative mitochondrial DNA damage in peripheral blood mononuclear cells is associated with reduced volumes of hippocampus and subcortical gray matter in chronically HIV-infected patients

Mitochondrion. 2016 May;28:8-15. doi: 10.1016/j.mito.2016.02.006. Epub 2016 Feb 23.


Cross-sectional relationships were examined between regional brain volumes and mitochondrial DNA (mtDNA) 8-hydroxy-2-deoxyguanosine (8-oxo-dG) in peripheral blood mononuclear cells (PBMCs) of 47 HIV patients [mean age 51years; 81% with HIV RNA ≤50copies/mL] on combination antiretroviral therapy. The gene-specific DNA damage and repair assay measured mtDNA 8-oxo-dG break frequency. Magnetic resonance imaging was performed at 3T. Higher mtDNA 8-oxo-dG was associated with lateral ventricular enlargement and with decreased volumes of hippocampus, pallidum, and total subcortical gray matter, suggesting the involvement of systemic mitochondrial-specific oxidative stress in chronic HIV-related structural brain changes and cognitive difficulties. Clarification of the mechanism may provide potential therapeutic targets.

Keywords: Atrophy; Brain; Neuroinflammation; Oxidative stress; Pallidum; ROS.

MeSH terms

  • 8-Hydroxy-2'-Deoxyguanosine
  • Adult
  • Atrophy / diagnostic imaging
  • Atrophy / pathology*
  • Cross-Sectional Studies
  • DNA Damage
  • DNA, Mitochondrial / chemistry*
  • Deoxyguanosine / analogs & derivatives*
  • Deoxyguanosine / analysis
  • Female
  • Gray Matter / diagnostic imaging
  • Gray Matter / pathology*
  • HIV Infections / complications*
  • Hippocampus / diagnostic imaging
  • Hippocampus / pathology*
  • Humans
  • Leukocytes, Mononuclear / pathology*
  • Magnetic Resonance Imaging
  • Male
  • Middle Aged
  • Oxidative Stress
  • Reactive Oxygen Species / toxicity


  • DNA, Mitochondrial
  • Reactive Oxygen Species
  • 8-Hydroxy-2'-Deoxyguanosine
  • Deoxyguanosine