Up-regulated expression of Ran reveals its potential role to deltamethrin stress in Kc cells

Gene. 2016 May 25;583(1):1-7. doi: 10.1016/j.gene.2016.02.037. Epub 2016 Feb 27.


The GTP-binding nuclear protein Ran has mostly been reported to be an essential player in nuclear transport, chromosome alignment, microtubule dynamics, centrosome duplication, kinetochore attachment of microtubules, nuclear-envelope dynamics, and phagocytosis. However, until now, there has been no report showing the involvement of Ran in DM stress. In this paper, two-dimensional electrophoresis analysis showed that the expression level of Ran in Kc cells in response to DM was higher than that in the control group. In addition, quantitative analysis using real-time PCR revealed that the expression of Ran was obviously up-regulated at various concentrations of DM. Western blot analysis showed that Ran was up-regulated 2.27-fold over the control at 48h. Because we still could not pinpoint whether Ran was actually involved in DM stress reaction, to further verify the role of Ran in stress reaction, RNA interference and cell transfection were utilized. Overexpression of Ran in cells conferred a degree of protection against DM after 72h. Furthermore, interference with Ran significantly decrease cell viability. All of the above findings strongly imply that Ran may participate in the development of stress reaction to DM. Therefore, investigating the possible role of Ran in DM stress will broaden our limited knowledge regarding DM stress inducible genes.

Keywords: Deltamethrin; Kc cells; Ran; Stress reaction.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Drosophila Proteins / genetics*
  • Drosophila Proteins / metabolism
  • Drosophila melanogaster / drug effects
  • Drosophila melanogaster / embryology
  • Drosophila melanogaster / genetics
  • Electrophoresis, Gel, Two-Dimensional
  • Embryo, Nonmammalian / cytology
  • Embryo, Nonmammalian / drug effects
  • Gene Expression Regulation / drug effects
  • Gene Knockdown Techniques
  • Inactivation, Metabolic
  • Insecticide Resistance / drug effects
  • Insecticide Resistance / genetics
  • Nitriles / pharmacology*
  • Pyrethrins / pharmacology*
  • Stress, Physiological / drug effects
  • Stress, Physiological / genetics*
  • Up-Regulation / drug effects
  • ran GTP-Binding Protein / genetics*
  • ran GTP-Binding Protein / metabolism


  • Drosophila Proteins
  • Nitriles
  • Pyrethrins
  • decamethrin
  • ran GTP-Binding Protein