Evaluation of Genetic and Phenotypic Consistency of Bacillus Coagulans MTCC 5856: A Commercial Probiotic Strain

World J Microbiol Biotechnol. 2016 Apr;32(4):60. doi: 10.1007/s11274-016-2027-2. Epub 2016 Feb 29.

Abstract

Commercial probiotics preparation containing Bacillus coagulans have been sold in the market for several decades. Due to its high intra-species genomic diversity, it is very likely that B. coagulans strain may alter in different ways over multiple years of production. Therefore, the present study focuses to evaluate the genetic consistency and probiotic potential of B. coagulans MTCC 5856. Phenotypic and genotypic techniques including biochemical profiling, 16S rRNA sequencing, GTG 5″, BOX PCR fingerprinting, and Multi-Locus-Sequence typing (MLST) were carried out to evaluate the identity and consistency of the B. coagulans MTCC 5856. Further, in vitro probiotic potential, safety and stability at ambient temperature conditions of B. coagulans MTCC 5856 were evaluated. All the samples were identified as B. coagulans by biochemical profiling and 16S rRNA sequencing. GTG 5″, BOX PCR fingerprints and MLST studies revealed that the same strain was present over 3 years of commercial production. B. coagulans MTCC 5856 showed resistance to gastric acid, bile salt and exhibited antimicrobial activity in in-vitro studies. Additionally, B. coagulans MTCC 5856 was found to be non-mutagenic, non-cytotoxic, negative for enterotoxin genes and stable at ambient temperature (25 ± 2 °C) for 36 months. The data of the study verified that the same strain of B. coagulans MTCC 5856 was present in commercial preparation over multiple years of production.

Keywords: 16S rRNA; Bacillus coagulans MTCC 5856; Genotypic fingerprinting; LactoSpore®; MLST.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacillus / classification*
  • Bacillus / genetics
  • Bacillus / physiology*
  • Bacterial Proteins / genetics*
  • Bacterial Typing Techniques / methods*
  • DNA Fingerprinting
  • DNA, Bacterial / genetics
  • DNA, Ribosomal / genetics
  • Multilocus Sequence Typing
  • Phenotype
  • Probiotics
  • RNA, Ribosomal, 16S / genetics

Substances

  • Bacterial Proteins
  • DNA, Bacterial
  • DNA, Ribosomal
  • RNA, Ribosomal, 16S