Abstract
Mutations in a known yeast gene, ADE3, were shown to act as an antisuppressor, reducing the efficiency of the omnipotent suppressor, sup45-2. The ADE3 locus encodes the trifunctional enzyme C1-tetrahydrofolate synthase, which is required for the biosynthesis of purines, thymidylate, methionine, histidine, pantothenic acid and formylmethionyl-tRNA(fMet. The role of this enzyme in translational fidelity had not previously been suspected.
Publication types
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Aminohydrolases / genetics*
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Cloning, Molecular
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DNA, Fungal / genetics
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Formate-Tetrahydrofolate Ligase / genetics*
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Genes, Fungal*
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Genetic Complementation Test
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Ligases / genetics*
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Methylenetetrahydrofolate Dehydrogenase (NADP) / genetics*
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Multienzyme Complexes / genetics*
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Mutation*
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Oxidoreductases / genetics*
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Phenotype
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Restriction Mapping
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Saccharomyces cerevisiae / enzymology
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Saccharomyces cerevisiae / genetics*
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Suppression, Genetic*
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Transformation, Genetic
Substances
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DNA, Fungal
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Multienzyme Complexes
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formyl-methenyl-methylenetetrahydrofolate synthetase
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Oxidoreductases
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Methylenetetrahydrofolate Dehydrogenase (NADP)
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Aminohydrolases
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Ligases
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Formate-Tetrahydrofolate Ligase