Mutations in ADE3 reduce the efficiency of the omnipotent suppressor sup45-2

Curr Genet. 1989 Dec;16(5-6):315-21. doi: 10.1007/BF00340709.

Abstract

Mutations in a known yeast gene, ADE3, were shown to act as an antisuppressor, reducing the efficiency of the omnipotent suppressor, sup45-2. The ADE3 locus encodes the trifunctional enzyme C1-tetrahydrofolate synthase, which is required for the biosynthesis of purines, thymidylate, methionine, histidine, pantothenic acid and formylmethionyl-tRNA(fMet. The role of this enzyme in translational fidelity had not previously been suspected.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Aminohydrolases / genetics*
  • Cloning, Molecular
  • DNA, Fungal / genetics
  • Formate-Tetrahydrofolate Ligase / genetics*
  • Genes, Fungal*
  • Genetic Complementation Test
  • Ligases / genetics*
  • Methylenetetrahydrofolate Dehydrogenase (NADP) / genetics*
  • Multienzyme Complexes / genetics*
  • Mutation*
  • Oxidoreductases / genetics*
  • Phenotype
  • Restriction Mapping
  • Saccharomyces cerevisiae / enzymology
  • Saccharomyces cerevisiae / genetics*
  • Suppression, Genetic*
  • Transformation, Genetic

Substances

  • DNA, Fungal
  • Multienzyme Complexes
  • formyl-methenyl-methylenetetrahydrofolate synthetase
  • Oxidoreductases
  • Methylenetetrahydrofolate Dehydrogenase (NADP)
  • Aminohydrolases
  • Ligases
  • Formate-Tetrahydrofolate Ligase