Growth properties of human herpesvirus-6 strain Z29

J Virol Methods. 1989 Nov;26(2):133-45. doi: 10.1016/0166-0934(89)90143-2.

Abstract

Experiments performed to optimize the growth conditions of HHV-6(Z29) revealed that the virus grows best in phytohemagglutinin-stimulated umbilical cord blood lymphocytes (CBL) cultured in media containing 32 units/ml interleukin-2 and 0.01 mg/ml hydrocortisone. The titer of maternal antibody in the plasma of the cord blood cells does not affect the ability of the cells to support virus growth. DEAE-dextran and polybrene do not increase virus growth in umbilical cord blood lymphocytes. Phorbol myristate acetate abolishes virus growth. The HHV-6(Z29) growth cycle in CBL was approximately 5 days; capsids were not seen before day 3, and mature virions were not seen before day 5.

MeSH terms

  • Cells, Cultured
  • Culture Media
  • DNA, Viral / analysis
  • Fetal Blood
  • Fluorescent Antibody Technique
  • Herpesviridae / drug effects
  • Herpesviridae / genetics
  • Herpesviridae / growth & development*
  • Herpesviridae / ultrastructure
  • Humans
  • Hydrocortisone / pharmacology
  • Interleukin-2 / pharmacology
  • Lymphocytes
  • Phytohemagglutinins / pharmacology
  • Virus Replication

Substances

  • Culture Media
  • DNA, Viral
  • Interleukin-2
  • Phytohemagglutinins
  • Hydrocortisone