Type 1 diabetes cadaveric human pancreata exhibit a unique exocrine tissue proteomic profile

Proteomics. 2016 May;16(9):1432-46. doi: 10.1002/pmic.201500333. Epub 2016 Apr 13.


Type 1 diabetes (T1D) is an autoimmune disorder resulting from a self-destruction of pancreatic islet beta cells. The complete proteome of the human pancreas, where both the dysfunctional beta cells and their proximal environment co-exist, remains unknown. Here, we used TMT10-based isobaric labeling and multidimensional LC-MS/MS to quantitatively profile the differences between pancreatic head region tissues from T1D (N = 5) and healthy subjects (N = 5). Among the 5357 (1% false discovery rate) confidently identified proteins, 145 showed statistically significant dysregulation between T1D and healthy subjects. The differentially expressed pancreatic proteome supports the growing notion of a potential role for exocrine pancreas involvement in T1D. This study also demonstrates the utility for this approach to analyze dysregulated proteins as a means to investigate islet biology, pancreatic pathology and T1D pathogenesis.

Keywords: Biomedicine; Isobaric labeling; Pancreas proteome; TMT10; Type 1 diabetes; nPOD.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Adolescent
  • Adult
  • Cadaver
  • Case-Control Studies
  • Chromatography, Liquid
  • Diabetes Mellitus, Type 1 / genetics*
  • Diabetes Mellitus, Type 1 / metabolism
  • Diabetes Mellitus, Type 1 / pathology
  • Female
  • Gene Expression Profiling
  • Gene Expression Regulation
  • Gene Ontology
  • Humans
  • Insulin-Secreting Cells / metabolism*
  • Insulin-Secreting Cells / pathology
  • Male
  • Metabolic Networks and Pathways / genetics*
  • Molecular Sequence Annotation
  • Proteome / genetics*
  • Proteome / metabolism
  • Staining and Labeling / methods
  • Tandem Mass Spectrometry


  • Proteome