Dystrophin in skeletal muscle. I. Western blot analysis using a monoclonal antibody

J Neurol Sci. 1989 Dec;94(1-3):125-36. doi: 10.1016/0022-510x(89)90223-2.


The value of analysing dystrophin on Western blots of skeletal muscle for the differential diagnosis of Xp21 muscular dystrophies is now fairly well established. Here we describe a sensitive system based on monoclonal antibodies to dystrophin. The specificity of the antibodies was established and experiments were undertaken to identify the source of dystrophin-related protein bands which were detected on blots of normal skeletal muscle. These investigations formed a necessary preliminary study to the application of the assay to samples of muscle obtained at biopsy from patients with Duchenne and Becker muscular dystrophy.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antibodies, Monoclonal*
  • Biopsy
  • Blotting, Western
  • Dystrophin
  • Humans
  • Molecular Weight
  • Muscle Proteins / immunology*
  • Muscle Proteins / isolation & purification
  • Muscles / metabolism*
  • Muscular Dystrophies / diagnosis*
  • Muscular Dystrophies / metabolism


  • Antibodies, Monoclonal
  • Dystrophin
  • Muscle Proteins