Description of Kibdelosporangium banguiense sp. nov., a novel actinomycete isolated from soil of the forest of Pama, on the plateau of Bangui, Central African Republic

Antonie Van Leeuwenhoek. 2016 May;109(5):685-95. doi: 10.1007/s10482-016-0669-6. Epub 2016 Mar 2.


A novel actinomycete strain F-240,109(T) from the MEDINA collection was isolated from a soil sample collected in the forest of Pama, on the plateau of Bangui, Central African Republic. The strain was identified according to its 16S rRNA gene sequence as a new member of the genus Kibdelosporangium, being closely related to Kibdelosporangium aridum subsp. aridum (98.6 % sequence similarity), Kibledosporangium phytohabitans (98.3 %), Kibdelosporangium aridum subsp. largum (97.7 %), Kibdelosporangium philippinense (97.6 %) and Kibledosporangium lantanae (96.9 %). In order to resolve its precise taxonomic status, the strain was characterised through a polyphasic approach. The strain is a Gram-stain positive, aerobic, non-motile and catalase-positive actinomycete characterised by formation of extensively branched substrate mycelia and sparse brownish grey aerial mycelia with sporangium-like globular structures. The chemotaxonomic characterisation of strain F-240,109(T) corroborated its affiliation into the genus Kibdelosporangium. The peptidoglycan contains meso-diaminopimelic acid; the major menaquinone is MK-9(H4); the phospholipid profile contains high amounts of phosphatidylethanolamine, hydroxyphosphatidylethanolamine, diphosphatidylglycerol and an unidentified phospholipid; and the predominant cellular fatty acid methyl esters are iso-C16:0, iso-C14:0, iso-C15:0 and 2OH iso-C16:0. However, some key phenotypic differences regarding to its close relatives and DNA-DNA hybridization values indicate that strain F-240,109(T) represents a novel Kibdelosporangium species, for which the name Kibdelosporangium banguiense sp. nov. is proposed. The type strain is strain F-240,109(T) (=DSM 46670(T), =LMG 28181(T)).

Keywords: 16S rRNA; Actinomycetes; Kibdelosporangium banguiense sp. nov.; Polyphasic taxonomy; Pseudonocardiaceae.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Actinomycetales / classification*
  • Actinomycetales / cytology
  • Actinomycetales / genetics
  • Actinomycetales / isolation & purification*
  • Bacterial Typing Techniques
  • Central African Republic
  • DNA, Bacterial / genetics
  • Forests*
  • Phenotype
  • Phylogeny
  • RNA, Ribosomal, 16S / genetics
  • Soil
  • Soil Microbiology*


  • DNA, Bacterial
  • RNA, Ribosomal, 16S
  • Soil