MiR-1284 modulates multidrug resistance of gastric cancer cells by targeting EIF4A1

Oncol Rep. 2016 May;35(5):2583-91. doi: 10.3892/or.2016.4643. Epub 2016 Feb 29.


Routine chemotherapy as an important treatment mode often can not be effective because of multidrug resistance (MDR). MicroRNA (miRNA) modulates the expression of a great number of genes, including MDR. In this study, the expression of miR-1284 was reduced in gastric cancer (GC) tissue specimens with metastasis and in vincristine-resistant (VCR) GC SGC7901 cells (SGC-7901/VCR) compared to that in the controls. Recombinant lentiviral vectors with miR-1284 led to the overexpression of miR-1284 mRNA and reversed the chemoresistance of SGC7901/VCR cells, promoted cell cycle arrested at the G0/G1 phase, accelerated drug-induced apoptosis, and decreased migration and invasiveness of SGC-7901/VCR. In addition, the overexpression of miR-1284 sensitized tumors to chemotherapy in vivo. Our data provide combined evidence that miR-1284 can heighten the expression of MYC and reduce the expression of JUN, MMP12, and EIF4A1 that was the direct target. In conclusion, miR-1284 can function as a new regulator to reduce GC MDR cells by targeting EIF4A1.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • 3' Untranslated Regions
  • Antineoplastic Agents, Phytogenic / pharmacology
  • Apoptosis
  • Base Sequence
  • Binding Sites
  • Cell Line, Tumor
  • Cell Movement
  • Drug Resistance, Multiple
  • Drug Resistance, Neoplasm*
  • Eukaryotic Initiation Factor-4A / genetics*
  • Eukaryotic Initiation Factor-4A / metabolism
  • Gene Expression Regulation, Neoplastic
  • Humans
  • MicroRNAs / physiology*
  • RNA Interference*
  • Stomach Neoplasms / drug therapy
  • Stomach Neoplasms / genetics*
  • Stomach Neoplasms / metabolism
  • Vincristine / pharmacology


  • 3' Untranslated Regions
  • Antineoplastic Agents, Phytogenic
  • MIRN1284 microRNA, human
  • MicroRNAs
  • Vincristine
  • Eukaryotic Initiation Factor-4A