Isolation of alpha-tubulin genes from the human malaria parasite, Plasmodium falciparum: sequence analysis of alpha-tubulin

Mol Microbiol. 1989 Nov;3(11):1501-10. doi: 10.1111/j.1365-2958.1989.tb00136.x.


As a step towards identifying exploitable differences between host and parasite at the molecular level, we have isolated and sequenced genomic clones encompassing an entire alpha-tubulin gene (designated alpha-tubulin I) from the human malaria parasite, Plasmodium falciparum. The gene, which contains two introns, encodes a product with a predicted length of 453 amino acid residues (50.3 kD). The protein sequence shows a high degree of homology to other alpha-tubulins, particularly that of the coccidian parasite, Toxoplasma gondii (94%), whose gene carries introns in identical positions. Only one copy of the alpha-tubulin I gene itself was found, although a second gene designated alpha-II was also identified which is closely related but which differs at both the nucleotide and amino acid sequence levels. The alpha-I and beta-tubulin genes were found to reside on different chromosomes.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Codon
  • DNA / genetics
  • Genes
  • Introns
  • Molecular Sequence Data
  • Plasmodium falciparum / genetics*
  • Restriction Mapping
  • Sequence Homology, Nucleic Acid
  • Tubulin / genetics*


  • Codon
  • Tubulin
  • DNA

Associated data

  • GENBANK/X15979