Pathogenesis of New Strains of Newcastle Disease Virus From Israel and Pakistan

Vet Pathol. 2016 Jul;53(4):792-6. doi: 10.1177/0300985815622972. Epub 2016 Mar 3.


In the past few years, Newcastle disease virus (NDV) strains with epizootic characteristics belonging to subgenotypes VIIi and XIIIb emerged in the Middle East and Asia. In this study, 2 NDV strains-1 representative of subgenotype VIIi isolated in Israel (Kvuzat/13) and 1 representative of subgenotype XIIIb isolated in Pakistan (Karachi/07)-were characterized by intracerebral pathogenicity index and detailed clinicopathologic assessment. The intracerebral pathogenicity index values for Kvuzat/13 and Karachi/07 were 1.89 and 1.85, respectively, classifying these strains as virulent by international standards. In 4-week-old White Leghorn chickens, both strains caused 100% mortality within 4 (Kvuzat/13) and 5 (Karachi/07) days postinfection. Histopathology and immunohistochemistry for NDV nucleoprotein showed that both strains had wide systemic distribution, especially targeting lymphoid organs and mucosa-associated lymphoid tissues in the respiratory and intestinal tracts. Results of the animal experiment confirm that both Kvuzat/13 and Karachi/07 are highly virulent and behaved as velogenic viscerotropic NDV strains.

Keywords: Newcastle disease virus; avian paramyxovirus serotype 1; chickens; fifth NDV panzootic; pathogenesis; pathogenicity; subgenotype VIIi; subgenotype XIIIb; velogenic viscerotropic NDV.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Animals
  • Chickens
  • Intestines / pathology
  • Intestines / virology
  • Israel
  • Lymphocytes / pathology
  • Lymphocytes / virology
  • Newcastle Disease / diagnosis*
  • Newcastle Disease / metabolism
  • Newcastle Disease / pathology
  • Newcastle Disease / virology
  • Newcastle disease virus / isolation & purification*
  • Newcastle disease virus / pathogenicity
  • Nucleoproteins / genetics
  • Nucleoproteins / metabolism
  • Pakistan
  • Poultry Diseases / diagnosis*
  • Poultry Diseases / metabolism
  • Poultry Diseases / pathology
  • Poultry Diseases / virology
  • Respiratory System / pathology
  • Respiratory System / virology
  • Viral Proteins / genetics
  • Viral Proteins / metabolism


  • Nucleoproteins
  • Viral Proteins
  • nucleoprotein, Newcastle disease virus