The Brewed Rice Vinegar Kurozu Increases HSPA1A Expression and Ameliorates Cognitive Dysfunction in Aged P8 Mice

PLoS One. 2016 Mar 4;11(3):e0150796. doi: 10.1371/journal.pone.0150796. eCollection 2016.


Kurozu is a traditional Japanese rice vinegar. During fermentation and aging of the Kurozu liquid in an earthenware jar over 1 year, a solid residue called Kurozu Moromi is produced. In the present study, we evaluated whether concentrated Kurozu or Kurozu Moromi could ameliorate cognitive dysfunction in the senescence-accelerated P8 mouse. Senescence-accelerated P8 mice were fed 0.25% (w/w) concentrated Kurozu or 0.5% (w/w) Kurozu Moromi for 4 or 25 weeks. Kurozu suppressed cognitive dysfunction and amyloid accumulation in the brain, while Kurozu Moromi showed a tendency to ameliorate cognitive dysfunction, but the effect was not significant. We hypothesize that concentrated Kurozu has an antioxidant effect; however, the level of lipid peroxidation in the brain did not differ in senescence-accelerated P8 mice. DNA microarray analysis indicated that concentrated Kurozu increased HSPA1A mRNA expression, a protein that prevents protein misfolding and aggregation. The increase in HSPA1A expression by Kurozu was confirmed using quantitative real-time PCR and immunoblotting methods. The suppression of amyloid accumulation by concentrated Kurozu may be associated with HSPA1A induction. However, concentrated Kurozu could not increase HSPA1A expression in mouse primary neurons, suggesting it may not directly affect neurons.

MeSH terms

  • Acetic Acid / pharmacology
  • Acetic Acid / therapeutic use*
  • Aging / pathology
  • Amyloid / metabolism
  • Animals
  • Antioxidants / metabolism
  • Brain / drug effects
  • Brain / metabolism
  • Brain / pathology
  • Cognition Disorders / blood
  • Cognition Disorders / drug therapy*
  • Cognition Disorders / physiopathology*
  • Feeding Behavior / drug effects
  • Gene Expression Regulation / drug effects
  • HSP70 Heat-Shock Proteins / genetics*
  • HSP70 Heat-Shock Proteins / metabolism
  • Mice
  • Oligonucleotide Array Sequence Analysis
  • Oryza / chemistry*
  • RNA, Messenger / genetics
  • RNA, Messenger / metabolism
  • Thiobarbituric Acid Reactive Substances / metabolism


  • Amyloid
  • Antioxidants
  • HSP70 Heat-Shock Proteins
  • Hsp70.3 protein, mouse
  • RNA, Messenger
  • Thiobarbituric Acid Reactive Substances
  • Acetic Acid

Grant support

The authors received no specific funding for this work.