Molecular epidemiology of rotavirus in four provinces of Angola before vaccine introduction

J Med Virol. 2016 Sep;88(9):1511-20. doi: 10.1002/jmv.24510. Epub 2016 Mar 11.


Angola is a sub-Saharan country in southern Africa highly affected by diarrhoeal disease with limited epidemiological data regarding etiologic agents. This study was performed during 2012-2013, prior to rotavirus vaccine introduction, with the objective to detect and characterize the rotavirus strains circulating in four provinces of the country: Huambo, Luanda, Zaire, and Cabinda. A high rotavirus detection rate (35%, 117/334) was observed. G1 was the most common G-genotype (83.6%), whereas P[8] (50.9%) followed by P[6] (38.8%) were the most common P-types. G1P[8] was identified as the predominant combination (50%), followed by the unusual G1P[6] (29.3%). Strains such G2P[4], G8P[6], G9P[6], and G12P[6] were also found in lower frequencies (5.2-1.7%). The P[6] strains did not cluster in the phylogenetic trees according to their geographic origin or even the corresponding G-genotype, suggesting a limited number of recent introductions and extensive reassortment events. Our results represent the first report on rotavirus genotype profiles in Angola, showing a wide circulation of the unusual genotype G1P[6], and underline the importance of RV surveillance after the vaccine introduction. J. Med. Virol. 88:1511-1520, 2016. © 2016 Wiley Periodicals, Inc.

Keywords: children; diarrhoeal disease; genotypes.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Africa, Northern / epidemiology
  • Africa, Southern / epidemiology
  • Angola / epidemiology
  • Antigens, Viral / genetics
  • Capsid Proteins / genetics
  • Child, Preschool
  • Democratic Republic of the Congo / epidemiology
  • Diarrhea / epidemiology
  • Diarrhea / virology*
  • Feces / virology
  • Female
  • Gastroenteritis / epidemiology
  • Gastroenteritis / virology
  • Genotype
  • Humans
  • Male
  • Molecular Epidemiology
  • RNA, Viral / genetics
  • Rotavirus / genetics*
  • Rotavirus Infections / epidemiology*
  • Rotavirus Infections / virology
  • Rotavirus Vaccines


  • Antigens, Viral
  • Capsid Proteins
  • RNA, Viral
  • Rotavirus Vaccines