Callyptide A, a new cytotoxic peptide from the Red Sea marine sponge Callyspongia species

Lamiaa A Shaala; Diaa T A Youssef; Sabrin R M Ibrahim; Gamal A Mohamed

doi:10.1080/14786419.2016.1155577

Callyptide A, a new cytotoxic peptide from the Red Sea marine sponge Callyspongia species

Nat Prod Res. 2016 Dec;30(24):2783-2790. doi: 10.1080/14786419.2016.1155577. Epub 2016 Mar 7.

Authors

Lamiaa A Shaala^{1

2}, Diaa T A Youssef³, Sabrin R M Ibrahim^{4

5}, Gamal A Mohamed^{3

6}

Affiliations

¹ a Natural Products Unit, King Fahd Medical Research Center , King Abdulaziz University , Jeddah , Saudi Arabia.
² b Suez Canal University Hospital , Suez Canal University , Ismailia , Egypt.
³ c Department of Natural Products and Alternative Medicine, Faculty of Pharmacy , King Abdulaziz Universrabiaity , Jeddah , Saudi Arabia.
⁴ d Department of Pharmacognosy and Medicinal Chemistry, College of Pharmacy , Taibah University , Al Madinah Al Munawwarah , Saudi Arabia.
⁵ e Faculty of Pharmacy, Department of Pharmacognosy , Assiut University , Assiut , Egypt.
⁶ f Faculty of Pharmacy, Department of Pharmacognosy, Assiut Branch , Al-Azhar University , Assiut , Egypt.

PMID: 26946937
DOI: 10.1080/14786419.2016.1155577

Abstract

In the course of our continuing efforts to allocate bioactive secondary metabolites from Red Sea marine invertebrates, we have investigated the sponge Callyspongia species. The cytotoxic dichloromethane fraction of the methanolic extract of the sponge afforded a new cytotoxic peptide named callyptide A (1). Its structure was determined by extensive 1D and 2D NMR (COSY, HSQC and HMBC) studies and high-resolution mass spectral determination. The configuration of the amino acids was determined by Marfey's analysis. Callyptide A was found to exhibit growth inhibitory activity when tested against different cancer cell lines.

Keywords: species; Red Sea sponge; callyptide A; cancer cell lines; cytotoxic activity.