Characterization of a novel qepA3 variant in Enterobacter aerogenes

Dongguo Wang; Xitian Huang; Jiayu Chen; Yonghua Mou; Yongxiao Qi

doi:10.1016/j.jmii.2016.01.001

Characterization of a novel qepA3 variant in Enterobacter aerogenes

J Microbiol Immunol Infect. 2017 Apr;50(2):254-257. doi: 10.1016/j.jmii.2016.01.001. Epub 2016 Feb 10.

Authors

Dongguo Wang¹, Xitian Huang², Jiayu Chen³, Yonghua Mou⁴, Yongxiao Qi⁵

Affiliations

¹ Department of Clinical Laboratories for Infectious Disease, Taizhou Municipal Hospital, Zhejiang, China; Institute of Molecular Diagnostics of Taizhou University, Zhejiang, China. Electronic address: wdgtzs@163.com.
² Department of Infectious Disease, Wenling People's Hospital, Zhejiang, China. Electronic address: hxt2380@163.com.
³ Department of Laboratory Medicine, Medical College of Taizhou University, Zhejiang, China; Institute of Molecular Diagnostics of Taizhou University, Zhejiang, China.
⁴ Department of Hepatobiliary Surgery, Taizhou Municipal Hospital, Zhejiang, China.
⁵ Department of Laboratory Medicine, Medical College of Taizhou University, Zhejiang, China.

PMID: 26947590
DOI: 10.1016/j.jmii.2016.01.001

Abstract

Five isolates harboring qepA were studied by polymerase chain reaction (PCR) amplification and relevant methods. One was determined to be a novel qepA3 from Enterobacter aerogenes, and four involved three qepA1 and one qepA2 determinants from Escherichia coli; the qepA3 changed five amino acids. These results characterized genetic structures A, B, C, D, and E.

Keywords: Enterobacter aerogenes; Escherichia coli; genetic structure; novel qepA3 variant gene; quinolone resistance.

MeSH terms

Anti-Infective Agents / pharmacology
Bacterial Proteins / genetics*
DNA, Bacterial / genetics
Drug Resistance, Bacterial
Enterobacter aerogenes / drug effects
Enterobacter aerogenes / genetics*
Enterobacter cloacae / drug effects
Enterobacter cloacae / genetics
Enterobacteriaceae Infections / microbiology
Escherichia coli / drug effects
Escherichia coli / genetics
Genes, Bacterial
Genetic Structures
Microbial Sensitivity Tests
Point Mutation
Polymerase Chain Reaction / methods
Quinolones / pharmacology

Substances

Anti-Infective Agents
Bacterial Proteins
DNA, Bacterial
Quinolones