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. 2015;5(81):66294-66300.
doi: 10.1039/C5RA09056C. Epub 2015 Aug 4.

"Pop-slide" Patterning: Rapid Fabrication of Microstructured PDMS Gasket Slides for Biological Applications

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"Pop-slide" Patterning: Rapid Fabrication of Microstructured PDMS Gasket Slides for Biological Applications

Ramesh Ramji et al. RSC Adv. .
Free PMC article

Abstract

We describe a "pop-slide" patterning approach to easily produce thin film microstructures on the surface of glass with varying feature sizes (3 μm - 250 μm) and aspect ratios (0.066 - 3) within 45 minutes. This low cost method does not require specialized equipment while allowing us to produce micro structured gasket layers for sandwich assays and could be readily applied to many biological applications.

Keywords: Cell differentiation; High throughput screening; Mechanobiology; Micropatterning; Micropillars; Microwells; Open microchannels; Proteomics; Secretomic analysis; Topographies.

Figures

Figure 1
Figure 1
(A) Schematic representation of pop-slide patterning methodology. (B) Photograph of PDMS microwells patterned on a microscope glass slide using this method. (C) Photograph of thin film PDMS microstructures
Figure 2
Figure 2
SEM images of PDMS microstructures patterned on a glass slide using pop-slide method. The master mold was prepared using a plastic photomask with 10 μm minimum resolution (see Materials and Methods). (A–C) Circular, triangular and square wells with aspect ratios between 0.066 and 0.33. (D–F) Circular, triangular and square pillars with aspect ratios between 0.066 and 0.5. Scale bar represents 50 μm.
Figure 3
Figure 3
(A–D) SEM images of different PDMS microstructures produced on a glass slide with master mold produced using a chrome mask (minimum feature resolution ~3 μm). Features in (C) range from 3 – 10 μm. (E) SEM image of rectangular wells with variable widths patterned on a glass slide (mold produced using a plastic mask with minimum resolution 10 μm). (F) PDMS microcanals (open microchannels) with serpentine features to help in mixing of fluids are easily micropatterned on the surface of glass.
Fig 4
Fig 4
(A) Fluorescent images of protein (FITC-BSA) spotted inside each well of a micro-well array patterned onto a glass slide. Schematic representation of a cell based assay with capture antibodies spotted/coated onto the micro-well array. (B) Fluorescent image showing live cells (stained with Calcein AM) attached inside micro-well array patterned onto a glass slide. Schematic representation of a cell-based assay with cells attached to the micro-well array and sandwiched under a capture antibody-coated slide. (C) 3T3 cells seeded on pop-slide patterned micro-pillars and micro-gratings for cell mechanistic studies. Cells are stained for nuclear and actin localization markers, DAPI and Phalloidin-647 respectively. (D) ECM – rhodamine-fibronectin coated on micro-pillar and micro-gratings produced by pop-slide patterning. Inserts shown in C and D represent magnified images as shown. Scale bars represent 50 μm.

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