CRISPR-Based Methods for Caenorhabditis elegans Genome Engineering

Genetics. 2016 Mar;202(3):885-901. doi: 10.1534/genetics.115.182162.


The advent of genome editing techniques based on the clustered regularly interspersed short palindromic repeats (CRISPR)-Cas9 system has revolutionized research in the biological sciences. CRISPR is quickly becoming an indispensible experimental tool for researchers using genetic model organisms, including the nematode Caenorhabditis elegans. Here, we provide an overview of CRISPR-based strategies for genome editing in C. elegans. We focus on practical considerations for successful genome editing, including a discussion of which strategies are best suited to producing different kinds of targeted genome modifications.

Keywords: CRISPR/Cas9; Caenorhabditis elegans; WormBook; genome editing.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Review

MeSH terms

  • Animals
  • CRISPR-Cas Systems*
  • Caenorhabditis elegans / genetics*
  • DNA Repair
  • Genetic Engineering / methods*
  • Mutagenesis, Site-Directed