High-quality genome sequence and description of Paenibacillus dakarensis sp. nov

C I Lo; S A Sankar; B Fall; B Sambe-Ba; O Mediannikov; C Robert; N Faye; B Wade; D Raoult; P-E Fournier; F Fenollar

doi:10.1016/j.nmni.2016.01.011

High-quality genome sequence and description of Paenibacillus dakarensis sp. nov

New Microbes New Infect. 2016 Jan 22:10:132-41. doi: 10.1016/j.nmni.2016.01.011. eCollection 2016 Mar.

Authors

C I Lo¹, S A Sankar¹, B Fall², B Sambe-Ba², O Mediannikov³, C Robert¹, N Faye⁴, B Wade², D Raoult⁵, P-E Fournier¹, F Fenollar³

Affiliations

¹ Aix-Marseille Université, URMITE, UM63, CNRS 7278, IRD 198, Inserm U1095, Faculté de médecine, Marseille, France.
² Hôpital Principal de Dakar, Senegal.
³ Aix-Marseille Université, URMITE, UM63, CNRS 7278, IRD 198, Inserm U1095, Faculté de médecine, Marseille, France; Campus International UCAD-IRD, Senegal.
⁴ Université Cheikh Anta Diop de Dakar, Laboratoire de Parasitologie générale, Dakar, Senegal.
⁵ Aix-Marseille Université, URMITE, UM63, CNRS 7278, IRD 198, Inserm U1095, Faculté de médecine, Marseille, France; Campus International UCAD-IRD, Senegal; Special Infectious Agents Unit, King Fahd Medical Research Center, King Abdulaziz University, Jeddah, Saudi Arabia.

Abstract

Strain FF9T was isolated in Dakar (Senegal) from a blood-culture taken from a 16-month-old child. MALDI-TOF analysis did not allow for identification. After sequencing, strain FF9T exhibited 98.18% similarity with the 16SrRNA sequence of Paenibacillus uliginis. A polyphasic study of phenotypic and genomic analyses showed that strain FF9T is Gram variable, catalase-positive, and presents a genome of 4,569,428 bp (one chromosome but no plasmid) with 4,427genes (4,352 protein-coding and 75 RNA genes (including 3 rRNA operons). The G+C content is 45.7%. On the basis of these genomic and phenotypic data analyses, we propose the creation of Paenibacillus dakarensis strain FF9T.

Keywords: Culturomics; Paenibacillus dakarensis; genome; taxono-genomics.