Histology Atlas of the Developing Mouse Hepatobiliary Hemolymphatic Vascular System with Emphasis on Embryonic Days 11.5-18.5 and Early Postnatal Development

Toxicol Pathol. 2016 Jul;44(5):705-25. doi: 10.1177/0192623316630836. Epub 2016 Mar 8.


A critical event in embryo development is the proper formation of the vascular system, of which the hepatobiliary system plays a pivotal role. This has led researchers to use transgenic mice to identify the critical steps involved in developmental disorders associated with the hepatobiliary vascular system. Vascular development is dependent upon normal vasculogenesis, angiogenesis, and the transformation of vessels into their adult counterparts. Any alteration in vascular development has the potential to cause deformities or embryonic death. Numerous publications describe specific stages of vascular development relating to various organs, but a single resource detailing the stage-by-stage development of the vasculature pertaining to the hepatobiliary system has not been available. This comprehensive histology atlas provides hematoxylin & eosin and immunohistochemical-stained sections of the developing mouse blood and lymphatic vasculature with emphasis on the hepatobiliary system between embryonic days (E) 11.5-18.5 and the early postnatal period. Additionally, this atlas includes a 3-dimensional video representation of the E18.5 mouse venous vasculature. One of the most noteworthy findings of this atlas is the identification of the portal sinus within the mouse, which has been erroneously misinterpreted as the ductus venosus in previous publications. Although the primary purpose of this atlas is to identify normal hepatobiliary vascular development, potential embryonic abnormalities are also described.

Keywords: atlas; embryo; hepatobiliary development; lymphatic development; mouse; portal sinus; vascular development.

Publication types

  • Research Support, N.I.H., Intramural

MeSH terms

  • Anatomy, Artistic
  • Animals
  • Atlases as Topic
  • Biliary Tract / embryology*
  • Blood Vessels / embryology*
  • Embryo, Mammalian
  • Embryonic Development
  • Liver / embryology*
  • Lymphatic System / embryology*
  • Mice
  • Mice, Transgenic