Survival and SOS response induction in ultraviolet B irradiated Escherichia coli cells with defective repair mechanisms

Int J Radiat Biol. 2016 Jun;92(6):321-8. doi: 10.3109/09553002.2016.1152412. Epub 2016 Mar 11.

Abstract

Purpose In this paper, the contribution of different genes involved in DNA repair for both survival and SOS induction in Escherichia coli mutants exposed to ultraviolet B radiation (UVB, [wavelength range 280-315 nm]) was evaluated. Materials and methods E. coli strains defective in uvrA, oxyR, recO, recN, recJ, exoX, recB, recD or xonA genes were used to determine cell survival. All strains also had the genetic sulA::lacZ fusion, which allowed for the quantification of SOS induction through the SOS Chromotest. Results Five gene products were particularly important for survival, as follows: UvrA > RecB > RecO > RecJ > XonA. Strains defective in uvrA and recJ genes showed elevated SOS induction compared with the wild type, which remained stable for up to 240 min after UVB-irradiation. In addition, E. coli strains carrying the recO or recN mutation showed no SOS induction. Conclusions The nucleotide excision and DNA recombination pathways were equally used to repair UVB-induced DNA damage in E. coli cells. The sulA gene was not turned off in strains defective in UvrA and RecJ. RecO protein was essential for processing DNA damage prior to SOS induction. In this study, the roles of DNA repair proteins and their contributions to the mechanisms that induce SOS genes in E. coli are proposed.

Keywords: DNA repair genes; Escherichia coli; SOS induction; UVB radiation.

MeSH terms

  • Bacterial Proteins / metabolism
  • Cell Survival / physiology
  • Cell Survival / radiation effects*
  • Dose-Response Relationship, Radiation
  • Escherichia coli / cytology
  • Escherichia coli / physiology*
  • Escherichia coli / radiation effects*
  • Radiation Dosage
  • SOS Response, Genetics / physiology*
  • SOS Response, Genetics / radiation effects*
  • Ultraviolet Rays*

Substances

  • Bacterial Proteins