Comparative studies on the effects of steroidal and nonsteroidal oestrogen antagonists on the proliferation of human breast cancer cells

J Steroid Biochem. 1989;34(1-6):183-8. doi: 10.1016/0022-4731(89)90081-2.


Pure and partial agonist antioestrogens, exemplified by ICI 164,384 and tamoxifen or 4-hydroxytamoxifen (4'-OHT) respectively, differ in their capacity to inhibit the growth of MCF-7 human breast cancer cells. Under basal conditions which maintain but do not permit proliferation of MCF-7 cells, growth rate was enhanced by oestradiol, Phenol Red, insulin and 4'-OHT but not by ICI 164,384. Oestradiol and insulin together enhanced cell growth rate synergistically. 4'-OHT, but not ICI 164,384, similarly increased insulin-stimulated cell growth in the absence of oestradiol. ICI 164,384 blocked the stimulatory action of 4'-OHT. The stimulatory effect of the peptide growth factors TGF-alpha and IGFI on MCF-7 cells were attenuated by ICI 164,384 and 4'-OHT; ICI 164,384 was more effective than 4'-OHT. The antiproliferative action of ICI 164,384 on oestradiol or growth factor stimulated MCF-7 cells was only weakly inhibited by TGF-beta antibodies.

Publication types

  • Comparative Study

MeSH terms

  • Breast Neoplasms
  • Cell Division / drug effects
  • Cell Line
  • Estradiol / analogs & derivatives
  • Estradiol / pharmacology*
  • Estrogen Antagonists / pharmacology*
  • Female
  • Humans
  • Insulin / pharmacology
  • Kinetics
  • Polyunsaturated Alkamides
  • Structure-Activity Relationship
  • Tamoxifen / analogs & derivatives
  • Tamoxifen / pharmacology
  • Tumor Cells, Cultured / cytology
  • Tumor Cells, Cultured / drug effects*


  • Estrogen Antagonists
  • Insulin
  • Polyunsaturated Alkamides
  • Tamoxifen
  • afimoxifene
  • Estradiol
  • ICI 164384