Bioaccumulation characterization of uranium by a novel Streptomyces sporoverrucosus dwc-3

Xiaolong Li; Congcong Ding; Jiali Liao; Liang Du; Qun Sun; Jijun Yang; Yuanyou Yang; Dong Zhang; Jun Tang; Ning Liu

doi:10.1016/j.jes.2015.06.007

Bioaccumulation characterization of uranium by a novel Streptomyces sporoverrucosus dwc-3

J Environ Sci (China). 2016 Mar:41:162-171. doi: 10.1016/j.jes.2015.06.007. Epub 2015 Aug 12.

Authors

Xiaolong Li¹, Congcong Ding², Jiali Liao³, Liang Du⁴, Qun Sun⁵, Jijun Yang⁶, Yuanyou Yang⁶, Dong Zhang⁴, Jun Tang⁶, Ning Liu⁷

Affiliations

¹ Key Laboratory of Radiation Physics and Technology, Ministry of Education, Institute of Nuclear Science and Technology, Sichuan University, Chengdu 610064, China. Electronic address: lixiaolongscu720@126.com.
² Key Laboratory of Radiation Physics and Technology, Ministry of Education, Institute of Nuclear Science and Technology, Sichuan University, Chengdu 610064, China; Key Laboratory of Biological Resource and Ecological Environment of the Ministry of Education, College of Life Sciences, Sichuan University, Chengdu 610064, China.
³ Key Laboratory of Radiation Physics and Technology, Ministry of Education, Institute of Nuclear Science and Technology, Sichuan University, Chengdu 610064, China. Electronic address: liaojiali@scu.edu.cn.
⁴ Institute of Nuclear Physics and Chemistry, CAEP, Mianyang 621900, China.
⁵ Key Laboratory of Biological Resource and Ecological Environment of the Ministry of Education, College of Life Sciences, Sichuan University, Chengdu 610064, China.
⁶ Key Laboratory of Radiation Physics and Technology, Ministry of Education, Institute of Nuclear Science and Technology, Sichuan University, Chengdu 610064, China.
⁷ Key Laboratory of Radiation Physics and Technology, Ministry of Education, Institute of Nuclear Science and Technology, Sichuan University, Chengdu 610064, China. Electronic address: nliu720@scu.edu.cn.

PMID: 26969062
DOI: 10.1016/j.jes.2015.06.007

Abstract

The biosorption mechanisms of uranium on an aerobic bacterial strain Streptomyces sporoverrucosus dwc-3, isolated from a potential disposal site for (ultra-)low uraniferous radioactive waste in Southwest China, were evaluated by using transmission electron microscopy (TEM), energy dispersive X-ray (EDX) analysis, Fourier transform infrared spectroscopy (FT-IR), X-ray photoelectron spectroscopy (XPS), proton induced X-ray emission (PIXE) and enhanced proton backscattering spectrometry (EPBS). Approximately 60% of total uranium at an initial concentration of 10mg/L uranium nitrate solution could be absorbed on 100mg S. sporoverrucosus dwc-3 with an adsorption capacity of more than 3.0mg/g (wet weight) after 12hr at room temperature at pH3.0. The dynamic biosorption process of S. sporoverrucosus dwc-3 for uranyl ions was well described by a pseudo second-order model. S. sporoverrucosus dwc-3 could accumulate uranium on cell walls and within the cell, as revealed by SEM and TEM analysis as well as EDX spectra. XPS and FT-IR analysis further suggested that the absorbed uranium was bound to amino, phosphate and carboxyl groups of the cells. Additionally, PIXE and EPBS results confirmed that ion exchange also contributed to the adsorption process of uranium.

Keywords: Bioaccumulation; Bioremediation; Nanoparticle; Streptomyces sporoverrucosus dwc-3; Uranium.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Biodegradation, Environmental
China
Environmental Monitoring
Soil Pollutants / metabolism*
Streptomyces / isolation & purification
Streptomyces / metabolism*
Uranium / metabolism*

Substances

Soil Pollutants
Uranium