Expression Profile and Tissue-Specific Distribution of the Receptor-Interacting Protein 3 in BALB/c Mice

Biochem Genet. 2016 Aug;54(4):360-367. doi: 10.1007/s10528-016-9724-2. Epub 2016 Mar 11.

Abstract

RIP3, a member of receptor-interacting protein family, is serine/threonine kinase that contributes to necrosis and promotes systematic inflammation. However, detailed information of the expression pattern and tissue distribution in BALB/c mice, a commonly used laboratory animal model, is still unavailable. Here, we provided the basic data of expression profile and histologic distribution of RIP3 in tissues of BALB/c mice. Rip3 mRNA expression levels and tissue distribution were detected by real-time quantitative PCR and immunohistochemical detection, respectively. Rip3 mRNA expression showed the highest level in the spleen and duodenum, while with the lowest level in brain. Immunohistochemical detection revealed this protein located in different type cells in different tissues. What's more, the obvious positive staining in nuclear was detected in liver cells and neurons in cerebral cortex of the brain, while cells in other organs, including heart, spleen, lung, kidney, stomach, duodenum and trachea, showed strong positive mainly in cytoplasm. The results will help us to further understand the site-specific functions of RIP3 in necrosis and inflammatory responses.

Keywords: BALB/c mice; Gene expression profile; RIP3; Tissue distribution.

MeSH terms

  • Animals
  • Brain / metabolism*
  • Cell Nucleus / metabolism
  • Cytoplasm / metabolism
  • Duodenum / metabolism*
  • Male
  • Mice
  • Mice, Inbred BALB C
  • Receptor-Interacting Protein Serine-Threonine Kinases / genetics*
  • Receptor-Interacting Protein Serine-Threonine Kinases / metabolism*
  • Spleen / metabolism*
  • Tissue Distribution
  • Transcriptome

Substances

  • Receptor-Interacting Protein Serine-Threonine Kinases
  • Ripk3 protein, mouse